RNA sequencing analysis provides new insights into dynamic molecular responses to Valsa mali pathogenicity in apple ‘Changfu No. 2’
RNA sequencing analysis provides new insights into dynamic molecular responses to Valsa mali pathogenicity in apple ‘Changfu No. 2’
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RNA测序分析为苹果“长富2号”致病性的动态分子反应提供了新见解
DOI：
10.1007/s11295-018-1288-3
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发表时间：
2018-09
影响因子：
2.4
通讯作者：
Baihong Chen
中科院分区：
文献类型：
--
作者：
Cunwu Zuo;Juan Mao;Zhongjian Chen;Mingyu Chu;Hu Duo;Baihong Chen
苹果腐烂病（Valsa mali，Vm）是一种严重危害东亚地区苹果生产的病害。苹果对Vm感染反应的分子基础知之甚少。因此，我们进行了RNA测序（RNA-seq），以调查一个主要的苹果品种，'长富2号'，在Vm感染过程中的动态基因表达谱。与对照（C）相比，在距病斑周边0.9- 1.3cm（T1）、0.5- 0.9cm（T2）和0.1- 0.5cm（T3）的韧皮部组织中分别检测到104、313和1059个差异表达基因（DEG）。基因本体富集分析表明，与植物生长发育相关的DEGs表达下调，而与防御反应相关的DEGs表达上调。京都基因和基因组百科全书（KEGG）分析表明，激素和Ca 2+信号以及苯丙烷类化合物的生物合成参与了防御反应。以上结果表明，“长辐2号”对Vm侵染的防御反应是由阿坝、JA、ET、Ca ~（2+）和细胞壁信号共同参与的。而表达被抑制的DEGs则参与植物的生长发育，生长素信号和多个抗病基因可能削弱了“长辐2号”对病原菌的抗性。我们的研究结果提供了一个新的见解植物对necrotrophs的反应，并可能有利于旨在为Vm抗性育种计划。
Valsa canker caused by the necrotrophic pathogen Valsa mali (Vm) severely affects apple production in Eastern Asia. The molecular basis underlying the apple response to Vm infection is poorly understood. Hence, we performed RNA sequencing (RNA-seq) to investigate the dynamic gene expression profiles of a major apple cultivar, ‘Changfu No.2’, during Vm infection. Compared with the control (C), 104, 313, and 1059 differentially expressed genes (DEGs) were detected from the phloem tissue within the range of 0.9–1.3 cm (T1), 0.5–0.9 cm (T2), and 0.1–0.5 cm (T3) beyond the lesion periphery, respectively. Gene ontology (GO) enrichment analysis revealed that the DEGs associated with plant growth and development were down-regulated, whereas those related to defense responses were up-regulated. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that hormonal and Ca2+ signaling and phenylpropanoid biosynthesis were involved in the defense responses. In conclusion, multiple defense responses associated with ABA, JA, ET, Ca2+, and cell wall signals contributed to the defense against Vm infection in ‘Changfu No.2’. In contrast, the DEGs with inhibited expression were involved in plant growth and development; auxin signaling and several resistance genes might weaken the resistance of ‘Changfu No.2’ to pathogens. Our results offer a new insight into plant responses against necrotrophs and could benefit programs aimed at breeding for Vm resistance.
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影响因子：
11.6
作者：
Lopez-Berges, Manuel S.;Rispail, Nicolas;Di Pietro, Antonio
通讯作者：
Di Pietro, Antonio
RNA测序揭示内质网应激和膜完整性破坏是二甲基三硫醚对尖孢镰刀菌的毒性的基础。
影响因子：
5.2
作者：
Zuo C;Zhang W;Chen Z;Chen B;Huang Y
通讯作者：
Huang Y
DOI：
10.1094/mpmi-21-10-1285
发表时间：
2008-10
期刊：
Molecular plant-microbe interactions : MPMI
影响因子：
--
作者：
Genger RK;Jurkowski GI;McDowell JM;Lu H;Jung HW;Greenberg JT;Bent AF
通讯作者：
Bent AF
影响因子：
14.9
作者：
Kanehisa M;Araki M;Goto S;Hattori M;Hirakawa M;Itoh M;Katayama T;Kawashima S;Okuda S;Tokimatsu T;Yamanishi Y
通讯作者：
Yamanishi Y
DOI：
--
发表时间：
2010
期刊：
--
影响因子：
--
作者：
JieLing Wei;H. Lili;ZuoPeng Gao;X. Ke;Zhen-sheng Kang
通讯作者：
JieLing Wei;H. Lili;ZuoPeng Gao;X. Ke;Zhen-sheng Kang