RNA Sequencing Reveals that Endoplasmic Reticulum Stress and Disruption of Membrane Integrity Underlie Dimethyl Trisulfide Toxicity against Fusarium oxysporum f. sp. cubense Tropical Race 4.
RNA Sequencing Reveals that Endoplasmic Reticulum Stress and Disruption of Membrane Integrity Underlie Dimethyl Trisulfide Toxicity against Fusarium oxysporum f. sp. cubense Tropical Race 4.
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RNA测序揭示内质网应激和膜完整性破坏是二甲基三硫醚对尖孢镰刀菌的毒性的基础。
DOI：
10.3389/fmicb.2017.01365
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发表时间：
2017
影响因子：
5.2
通讯作者：
Huang Y
中科院分区：
文献类型：
--
作者：
Zuo C;Zhang W;Chen Z;Chen B;Huang Y
香蕉枯萎病是由香蕉枯萎病菌引起的一种毁灭性病害。sp. cubense热带小种4（Foc TR 4）。在以前的研究中，我们证实了中国韭菜（韭菜）对这种疾病的发病率有很强的抑制作用。含硫化合物是韭菜抗真菌的主要成分。其中，二甲基三硫（DT）是最丰富的，并表现出最强的抑制Foc TR 4的生长和发育。在本研究中，通过使用RNA测序来研究用4，000倍稀释度（浓度为1/4，000，v/v）的DT处理1.5、6和12 h的Foc TR 4分离株的全局基因表达谱。采用实时荧光定量PCR（qRT-PCR）方法对15个DEG的表达模式进行验证。在实验开始后1.5、6和12 h，未处理的样品分别呈现2，556、1，691和1，150个差异表达基因（DEG），而DT处理的分离株呈现2，823、3，546和6，197个DEG。基于基因本体（GO）注释和京都基因与基因组百科全书（KEGG）富集分析，DT暴露显著抑制了参与内质网（ER）、糖基化和类固醇生物合成的DEG。RNA-seq和qRT-PCR检测之间15个DEG的相似表达模式表明RNA-seq数据的可靠性。总之，与糖基化抑制和细胞膜完整性损伤相关的ER应激可能有助于DT对Foc TR 4的毒性。本文的研究结果证实了DT暴露相关基因表达的变化，这可能用于开发控制FWB的新方法。
Fusarium wilt of banana, a destructive disease that affects banana production, is caused by Fusarium oxysporum f. sp. cubense tropical race 4 (Foc TR4). In a previous study, we confirmed the strong inhibitory effects of Chinese leek (Allium tuberosum) on the incidence of this disease. Sulfur compounds are the primary antifungal constituents of Chinese leek. Among these, dimethyl trisulfide (DT) was the most abundant and exhibited the strongest inhibition of Foc TR4 growth and development. In the present study, the global gene expression profiles of Foc TR4 isolates treated with DT at 4,000-folds dilution (concentration of 1/4,000, v/v) for 1.5, 6, and 12 h were investigated by using RNA sequencing. The expression patterns of 15 DEGs were validated based on quantitative real-time PCR (qRT-PCR) assay. Untreated sample presented 2,556, 1,691, and 1,150 differentially expressed genes (DEGs) at 1.5, 6, and 12 h after the onset of the experiment, respectively, whereas DT-treated isolates presented 2,823, 3,546, and 6,197 DEGs. Based on Gene Ontology (GO) annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis, DEGs involved in endoplasmic reticulum (ER), glycosylation, and steroid biosynthesis were significantly inhibited by DT exposure. The similar expressional patterns of 15 DEGs between RNA-seq and qRT-PCR assays indicated the reliability of the RNA-seq data. In conclusion, ER stress related to glycosylation inhibition and damage to cell membrane integrity might contribute to the toxicity of DT against Foc TR4. As the results presented here evidenced changes in gene expression associated with DT exposure, which might be used to develop new approaches for controlling FWB.
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影响因子：
14.9
作者：
Kanehisa M;Araki M;Goto S;Hattori M;Hirakawa M;Itoh M;Katayama T;Kawashima S;Okuda S;Tokimatsu T;Yamanishi Y
通讯作者：
Yamanishi Y
影响因子：
4.4
作者：
Fourie, Gerda;Steenkamp, E. T.;Viljoen, A.
通讯作者：
Viljoen, A.
影响因子：
1.8
作者：
Huang YH;Wang RC;Li CH;Zuo CW;Wei YR;Zhang L;Yi GJ
通讯作者：
Yi GJ
影响因子：
4.5
作者：
Hwang, SC;Ko, WH
通讯作者：
Ko, WH
影响因子：
2.1
作者：
Cao, LX;Qiu, ZQ;Zhou, SN
通讯作者：
Zhou, SN