Human papillomavirus 16 E7 oncoprotein alters the expression profiles of circular RNAs in Caski cells.
Human papillomavirus 16 E7 oncoprotein alters the expression profiles of circular RNAs in Caski cells.
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人乳头瘤病毒 16 E7 癌蛋白改变 Caski 细胞中环状 RNA 的表达谱
DOI：
10.7150/jca.24253
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发表时间：
2018
影响因子：
3.9
通讯作者：
Zhou JY
中科院分区：
文献类型：
--
作者：
Zheng SR;Zhang HR;Zhang ZF;Lai SY;Huang LJ;Liu J;Bai X;Ding K;Zhou JY
宫颈癌是全世界女性最常见的癌症之一。高危人乳头瘤病毒E7癌基因的表达对于恶性表型的维持和转化是必需的。人们对该蛋白在宫颈癌中的研究不断增多，但关于E7表达如何影响整体环状RNA表达的研究较少。 CircRNA作为一种很有前景的生物标志物甚至治疗靶点，已成为继miRNA和长链非编码RNA之后研究领域的明星分子。我们本研究的目的是调查 HPV E7 表达调节的全局 circRNA 水平，并确定机制研究的潜在后果。在这里，我们通过高通量微阵列技术在Caski细胞中转染E7 siRNA来研究circRNA的表达谱。总共，我们鉴定了 526 个失调的 circRNA，其倍数变化≥2 或≤0.5，且 p<0.05。其中，上调352个，下调174个。此外，使用qRT-PCR确认的8个选定的circRNA与微阵列分析的结果一致。此外，生物信息学分析表明，不同表达的circRNA可能与mTOR信号通路、脯氨酸代谢和谷胱甘肽代谢有关。总之，本研究展示了宫颈癌细胞中受 HPV16 E7 调控的 circRNA 的表达谱，并为未来机制研究的新潜在候选者提供了新的见解。
Cervical cancer is one of the most common cancer in female worldwide. The expression of high-risk human papillomavirus E7 oncogene is necessary for the maintenance of malignant phenotypes and transformation. Accumulating studies of this protein has been explored in cervical cancer, however, there are fewer studies on how E7 expression affects the expression of global circular RNA. CircRNA, a promising biomarker and even therapeutic target, has become a star molecular in research after miRNA and long non-coding RNA. Our aim of this study was to investigate the global circRNA levels modulated by HPV E7 expression and identified the potential consequences for mechanism studies. Here we investigated the expression profiles of circRNAs by transfecting E7 siRNA in Caski cells with high-throughput microarray technology. In total, we identified 526 dysregulated circRNAs with fold change ≥2 or≤0.5, and p< 0.05. Among them, 352 were up-regulated and 174 were down-regulated. In addition, 8 selected circRNAs confirmed using qRT-PCR was in line with the results of microarray analysis. Furthermore, bioinformatic analyses indicated that differently expressed circRNAs might implicate in the mTOR signaling pathway, proline metabolism and glutathione metabolism. In conclusion, this study showed the expression profiles of circRNAs regulated by HPV16 E7 in cervical cancer cells and provides novel insights into the new potential candidates for future mechanism studies.
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影响因子：
--
作者：
Gómez-Gómez Y;Organista-Nava J;Gariglio P
通讯作者：
Gariglio P
影响因子：
--
作者：
Gao YL;Zhang MY;Xu B;Han LJ;Lan SF;Chen J;Dong YJ;Cao LL
通讯作者：
Cao LL
影响因子：
5
作者：
Greene J;Baird AM;Brady L;Lim M;Gray SG;McDermott R;Finn SP
通讯作者：
Finn SP
影响因子：
3.7
作者：
Pereira PM;Marques JP;Soares AR;Carreto L;Santos MA
通讯作者：
Santos MA
影响因子：
37.3
作者：
Meng S;Zhou H;Feng Z;Xu Z;Tang Y;Li P;Wu M
通讯作者：
Wu M