Expression of intrinsic factor in rat and murine gastric mucosal cell lineages is modified by inflammation.
Expression of intrinsic factor in rat and murine gastric mucosal cell lineages is modified by inflammation.
复制标题
炎症改变大鼠和小鼠胃粘膜细胞谱系中内因子的表达。
DOI：
10.1016/s0002-9440(10)64635-4
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
Alpers,DH
中科院分区：
文献类型：
--
作者：
Shao,J;Sartor,RB;Dial,E;Lichtenberger,LM;Schepp,W;Alpers,DH
内因子主要由大鼠和小鼠的主细胞产生，但4 - 11%的分离大鼠壁细胞也含有内因子。为了测试局部条件是否可以改变内在因子表达的分布，检查了两种慢性淋巴细胞性胃炎的啮齿动物模型。使用抗人内因子和H/K ATP酶（壁细胞标记物）的抗血清进行免疫细胞化学，计数内因子阳性壁细胞的百分比。HLA-B27转基因大鼠在3月龄时发生慢性胃炎。同源对照组壁细胞内因子表达率为8.9 ± 3.8%（平均值±标准差），转基因大鼠炎症区壁细胞内因子表达率为21 ± 5.2%（P < 0.0001）。在无炎性浸润的邻近区域，16 ± 3.6%的壁细胞含有内源性因子。接种猫螺杆菌的C57 BL/6小鼠在4周时发生胃炎。感染4周和8周后，内源性因子阳性壁细胞从同类对照组的7.8 ± 2.8%增加到炎症胃体的17.6 ± 4.1%（P < 0.0001）。与白细胞介素-1 β孵育的分离大鼠壁细胞显示内在因子阳性壁细胞增加两倍。这些研究与内在因子表达在主细胞中是预先确定的并且可以在壁细胞中响应于局部炎症因子而表达的概念一致。大鼠模型中炎症和相邻非炎症区域之间的差异表明可溶性诱导物（可能是细胞因子）的组织梯度。
Intrinsic factor is produced primarily by chief cells in rat and mouse, but 4 to 11% of isolated rat parietal cells also contain intrinsic factor. To test whether local conditions could alter the distribution of intrinsic factor expression, two rodent models of chronic lymphocytic gastric inflammation were examined. Immunocytochemistry was performed using antiserum against human intrinsic factor and H/K ATPase (a parietal cell marker), counting the percent of intrinsic factor-positive parietal cells. HLA-B27 transgenic rats develop chronic gastritis at age 3 months. Congenic controls expressed intrinsic factor in 8.9 ± 3.8% (mean ± SD) of parietal cells; in inflamed areas of transgenic rats 21 ± 5.2% (P < 0.0001) of parietal cells were positive. In adjacent areas without inflammatory infiltrate 16 ± 3.6% of parietal cells contained intrinsic factor. C57BL/6 mice inoculated with Helicobacter felis develop gastritis by 4 weeks. After 4 and 8 weeks of infection, intrinsic factor-positive parietal cells increased from 7.8 ± 2.8% in the congenic controls to 17.6 ± 4.1% in the inflamed gastric body (P < 0.0001). Isolated rat parietal cells incubated with interleukin-1β demonstrated a twofold increase in intrinsic factor-positive parietal cells. These studies are consistent with the concept that intrinsic factor expression is both predetermined in chief cells and can be expressed in parietal cells in response to local inflammatory factors. The differences between inflamed and adjacent noninflamed areas in the rat model suggest a tissue gradient of soluble inducer(s), possibly cytokines.
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影响因子：
29.4
作者：
L. Maiuri;V. Raia;J. Potter;D. Swallow;M. Ho;R. Fiocca;G. Finzi;M. Cornaggia;C. Capella;A. Quaroni;S. Auricchio
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DOI：
--
发表时间：
1993
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
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通讯作者：
Gordon,JI
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影响因子：
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作者：
M. Serrano;A. Lanas;S. Lorente;R. Sáinz
通讯作者：
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影响因子：
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作者：
J. Levine;P. Nakane;R. Allen
通讯作者：
R. Allen
DOI：
10.1152/ajpgi.1998.275.5.g1094
发表时间：
1998-11-01
影响因子：
4.5
作者：
Schepp, W;Dehne, K;Prinz, C
通讯作者：
Prinz, C