Hepatic glucagon receptors expression in response to glucose, insulin, glucagon and immune challenge in grass carp (Ctenophcuyngodon idellus)
Hepatic glucagon receptors expression in response to glucose, insulin, glucagon and immune challenge in grass carp (Ctenophcuyngodon idellus)
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草鱼（Ctenophcuyngodon idellus）对葡萄糖、胰岛素、胰高血糖素和免疫攻击的肝胰高血糖素受体表达
DOI：
10.1016/j.aqrep.2020.100470
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发表时间：
2020-11
影响因子：
3.7
通讯作者：
Nie Guoxing
中科院分区：
文献类型：
--
作者：
Qin Chaobin;Zhao Wenli;Yang Guokun;Cao Hui;Yang Liping;Meng Xiaolin;Yan Xiao;Nie Guoxing
自从发现以来，胰高血糖素的生物学一直是个谜。研究表明，胰升糖素可能通过与其受体(GCGR)结合，具有超出葡萄糖调节的生理作用。胰升糖素及其受体在鱼类葡萄糖代谢和免疫功能之间的相互作用仍有待研究。在本研究中，我们对草鱼和草鱼进行了测序和鉴定。系统发育分析表明，它们与鱼类中的同源基因高度同源。Gcgras和gcgrbmRNAs在所有被检测的组织中普遍表达。该基因在草鱼肝脏中高表达，主要在肝脏和脑组织中表达。此外，口服葡萄糖耐量试验(OGTT)结果显示，两种受体的表达水平在治疗后12h显著增加。在肝细胞中，胰岛素分别在3−12h和6−12h抑制gcgrab和gcgrbmRNA的表达。而胰高血糖素分别在3−、12 h和3 h促进其表达。腹腔注射脂多糖(LPS)后6h，肝组织中的gcgrbRNA水平升高，24 h时，肝组织中gcgrbmRNA的丰度升高，但对多肌苷多胞酸刺激后，gcgrab和gcgrbmRNA的表达水平降低。这些结果表明，高血糖素受体除了在草鱼的葡萄糖代谢中发挥作用外，还受到细菌样和病毒样感染的影响。
Since discovery, glucagon biology has remained enigmatic. Studies have shown that glucagon may have physiological effects beyond glucose regulation by binding to its receptor (Gcgr). The potential roles of glucagon and its receptor in the crosstalk between glucose metabolism and immune function of fish remain to be investigated. In the present study, we sequenced and characterizedgcgraandgcgrbin grass carp. Phylogenetic analysis showed that they were highly homologous with their counterparts in fish. ThegcgraandgcgrbmRNAs were ubiquitously expressed in all the tissues examined. ThegcgramRNA was highly expressed in the liver andgcgrbwas mainly expressed in the liver and brain regions of grass carp. Furthermore, the results of oral glucose tolerance test (OGTT) showed that the expression levels of the two receptors were significantly increased at 12 h after treatment. In the hepatocytes, insulin inhibitedgcgraandgcgrbmRNA expressions at 3−12 h and 6−12 h, respectively. While glucagon promoted their expressions at 3−12 h and 3 h, respectively. Intraperitoneal injection of lipopolysaccharide (LPS) increased hepaticgcgramRNA level at 6 h post treatment, and up-regulatedgcgrbmRNA abundance in the liver at 24 h. In response to polyinosinic polycytidylic acid [poly (I:C)] stimuli, however,gcgraandgcgrbmRNA levels were decreased. These results suggested that glucagon receptors were affected by bacterial-like and virus-like infection beyond their roles in the glucose metabolism of grass carp.
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DOI：
10.1093/bioinformatics/8.3.275
发表时间：
1992-06-01
期刊：
COMPUTER APPLICATIONS IN THE BIOSCIENCES
影响因子：
--
作者：
JONES, DT;TAYLOR, WR;THORNTON, JM
通讯作者：
THORNTON, JM
调节健康和疾病中肝葡萄糖代谢。
DOI：
10.1038/nrendo.2017.80
发表时间：
2017-10
期刊：
Nature reviews. Endocrinology
影响因子：
--
作者：
Petersen MC;Vatner DF;Shulman GI
通讯作者：
Shulman GI
影响因子：
12.3
作者：
通讯作者：
--
影响因子：
3.4
作者：
Cardoso JC;Vieira FA;Gomes AS;Power DM
通讯作者：
Power DM
影响因子：
3.7
作者：
Oren DA;Wei Y;Skrabanek L;Chow BK;Mommsen T;Mojsov S
通讯作者：
Mojsov S