Inhibitory activity spectrum of reuterin produced by Lactobacillus reuteri against intestinal bacteria.
Inhibitory activity spectrum of reuterin produced by Lactobacillus reuteri against intestinal bacteria.
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DOI：
10.1186/1471-2180-7-101
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发表时间：
2007-11-12
期刊：
影响因子：
4.2
通讯作者：
Le Blay G
中科院分区：
文献类型：
--
作者：
Cleusix V;Lacroix C;Vollenweider S;Duboux M;Le Blay G
鲁氏乳杆菌是人体肠道的正常居留者，由甘油产生的芦丁是一种广谱抗菌剂。有人推测，reurin可能在lb的益生菌效应中发挥作用。路易。Reurin对肠道病原体、酵母、真菌、原生动物和病毒具有活性，但其对共生肠道细菌的影响尚不清楚。此外，reurin的作用模式尚未阐明。谷胱甘肽是一种强大的抗氧化剂，它在解毒活性醛方面也起着关键作用，它可以保护某些细菌免受氧化应激的伤害，也可能与对芦丁的抗药性有关。这项工作的目的是测试reurin对一组具有代表性的肠道细菌的活性，并研究细胞内低分子硫醇(LMW-SH)(如谷胱甘肽、过氧化氢和/或reurin敏感性)之间的可能相关性。路宁是由lb生产的。Reuri SD2112在纯甘油溶液中提纯，用于测试最小抑菌浓度(MIC)和最小杀菌浓度(MBC)。并对过氧化氢敏感性和胞内LMW-SH浓度进行了分析。我们的数据显示，大多数测试的肠道细菌的MIC低于敏感指标大肠杆菌(7.5-15 mm)的MIC。乳杆菌和梭状芽孢杆菌对15~50 mm的MIC耐药率较高。未发现细菌内LMW-SH浓度(包括谷胱甘肽)与尿嘧啶或过氧化氢敏感性之间的相关性。我们的数据显示，肠道细菌对reurin非常敏感，它们细胞内的LMW-SH浓度与它们抵抗reurin或过氧化氢的能力没有直接联系。这表明，谷胱甘肽等LMW-SH的解毒作用不是一般的机制，其他机制可能与细菌对腐胺和氢基因过氧化氢的耐受性有关。
Reuterin produced from glycerol by Lactobacillus reuteri, a normal inhabitant of the human intestine, is a broad-spectrum antimicrobial agent. It has been postulated that reuterin could play a role in the probiotic effects of Lb. reuteri. Reuterin is active toward enteropathogens, yeasts, fungi, protozoa and viruses, but its effect on commensal intestinal bacteria is unknown. Moreover reuterin's mode of action has not yet been elucidated. Glutathione, a powerful antioxidant, which also plays a key role in detoxifying reactive aldehydes, protects certain bacteria from oxidative stress, and could also be implicated in resistance to reuterin. The aim of this work was to test the activity of reuterin against a representative panel of intestinal bacteria and to study a possible correlation between intracellular low molecular weight thiols (LMW-SH) such as glutathione, hydrogen peroxide and/or reuterin sensitivity. Reuterin was produced by Lb. reuteri SD2112 in pure glycerol solution, purified and used to test the minimal inhibitory (MIC) and minimal bactericidal concentrations (MBC). Hydrogen peroxide sensitivity and intracellular LMW-SH concentration were also analysed. Our data showed that most tested intestinal bacteria showed MIC below that for a sensitive indicator Escherichia coli (7.5–15 mM). Lactobacilli and Clostridium clostridioforme were more resistant with MIC ranging from 15 to 50 mM. No correlation between bacterial intracellular concentrations of LMW-SH, including glutathione, and reuterin or hydrogen peroxide sensitivities were found. Our data showed that intestinal bacteria were very sensitive to reuterin and that their intracellular concentration of LMW-SH was not directly linked to their capacity to resist reuterin or hydrogen peroxide. This suggests that detoxification by LMW-SH such as glutathione is not a general mechanism and that other mechanisms are probably involved in bacterial tolerance to reuterin and hydrogene peroxide.
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DOI：
10.1021/i560140a021
发表时间：
1945-01-01
影响因子：
--
作者：
CIRCLE, SJ;STONE, L;BORUFF, CS
通讯作者：
BORUFF, CS
DOI：
10.1111/j.1574-695x.2002.tb00541.x
发表时间：
2002-01-14
影响因子：
--
作者：
Mukai, T;Asasaka, T;Ohori, H
通讯作者：
Ohori, H
DOI：
10.1016/s1383-5718(99)00052-2
发表时间：
1999-06-07
影响因子：
1.9
作者：
Nunoshiba, T;Yamamoto, K
通讯作者：
Yamamoto, K
影响因子：
7.1
作者：
Holzapfel, WH;Haberer, P;Schillinger, U
通讯作者：
Schillinger, U
影响因子：
2.7
作者：
El-Ziney, MG;Arneborg, N;Jakobsen, M
通讯作者：
Jakobsen, M