Glia Cell Morphology Analysis Using the Fiji GliaMorph Toolkit.
Glia Cell Morphology Analysis Using the Fiji GliaMorph Toolkit.
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DOI：
10.1002/cpz1.654
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发表时间：
2023-01
期刊：
影响因子：
--
通讯作者：
MacDonald, Ryan
中科院分区：
文献类型：
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作者：
Kugler, Elisabeth;Breitenbach, Eva-Maria;MacDonald, Ryan
神经胶质细胞是神经系统的支持细胞。神经胶质细胞通常具有复杂的形态，有助于与邻近的神经元、突触和脉管系统密切接触。在视网膜中，突触神经胶质（MG）是支持神经元功能的主要胶质细胞类型，通过复杂的细胞形态和精确的接触提供无数的支持功能。因此，复杂的胶质细胞形态对胶质细胞功能至关重要，但在亚细胞水平上解决或在复杂组织中可重复量化仍然具有挑战性。为了解决这个问题，我们开发了GliaMorph作为一个基于斐济的宏观工具包，允许在发育和成熟的视网膜中进行3D胶质细胞形态分析。由于GliaMorph是以模块化的方式实现的，在这里我们提供了以下指南：(a) GliaMorph的设置，(b) 3D数据理解，包括z轴强度衰减和信噪比，(c)预处理数据以增强图像质量，(d)执行和检查图像分割，以及(e) MG特征的3D量化，包括尖基底纹理分析。为了简化应用程序，GliaMorph工具在适当的地方提供图形用户界面支持，并公开提供示例数据以促进采用。此外，GliaMorph可以修改以满足用户对其他胶质或神经元形状的形态学分析需求。最后，本文为用户提供了对数据需求和GliaMorph工作流程的深入了解。©2023作者。Wiley期刊有限责任公司发布的当前协议基本协议1：下载和安装GliaMorph组件，包括示例数据基本协议2：理解数据属性和质量3d -对后续分析和捕获数据属性问题早期至关重要基本协议3：预处理airscan显微镜数据用于分析备用协议：预处理共聚焦显微镜数据用于分析基本协议4：胶质细胞分割基本协议5：胶质细胞形态的三维定量
Glial cells are the support cells of the nervous system. Glial cells typically have elaborate morphologies that facilitate close contacts with neighboring neurons, synapses, and the vasculature. In the retina, Müller glia (MG) are the principal glial cell type that supports neuronal function by providing a myriad of supportive functions via intricate cell morphologies and precise contacts. Thus, complex glial morphology is critical for glial function, but remains challenging to resolve at a sub‐cellular level or reproducibly quantify in complex tissues. To address this issue, we developed GliaMorph as a Fiji‐based macro toolkit that allows 3D glial cell morphology analysis in the developing and mature retina. As GliaMorph is implemented in a modular fashion, here we present guides to (a) setup of GliaMorph, (b) data understanding in 3D, including z‐axis intensity decay and signal‐to‐noise ratio, (c) pre‐processing data to enhance image quality, (d) performing and examining image segmentation, and (e) 3D quantification of MG features, including apicobasal texture analysis. To allow easier application, GliaMorph tools are supported with graphical user interfaces where appropriate, and example data are publicly available to facilitate adoption. Further, GliaMorph can be modified to meet users’ morphological analysis needs for other glial or neuronal shapes. Finally, this article provides users with an in‐depth understanding of data requirements and the workflow of GliaMorph. © 2023 The Authors. Current Protocols published by Wiley Periodicals LLC. Basic Protocol 1: Download and installation of GliaMorph components including example data Basic Protocol 2: Understanding data properties and quality 3D—essential for subsequent analysis and capturing data property issues early Basic Protocol 3: Pre‐processing AiryScan microscopy data for analysis Alternate Protocol: Pre‐processing confocal microscopy data for analysis Basic Protocol 4: Segmentation of glial cells Basic Protocol 5: 3D quantification of glial cell morphology
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DOI：
10.1083/jcb.201004104
发表时间：
2010-05-31
期刊：
The Journal of cell biology
影响因子：
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作者：
Linkert M;Rueden CT;Allan C;Burel JM;Moore W;Patterson A;Loranger B;Moore J;Neves C;Macdonald D;Tarkowska A;Sticco C;Hill E;Rossner M;Eliceiri KW;Swedlow JR
通讯作者：
Swedlow JR
影响因子：
2.6
作者：
Parsons, Michael J.;Pisharath, Harshan;Yusuff, Shamila;Moore, John C.;Siekmann, Arndt F.;Lawson, Nathan;Leach, Steven D.
通讯作者：
Leach, Steven D.
影响因子：
4.6
作者：
Koho S;Fazeli E;Eriksson JE;Hänninen PE
通讯作者：
Hänninen PE
影响因子：
5.5
作者：
Angueyra JM;Kindt KS
通讯作者：
Kindt KS
DOI：
10.1242/dev.201008
发表时间：
2023-02-15
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
通讯作者：
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