The Biology of Veganism: Plasma Metabolomics Analysis Reveals Distinct Profiles of Vegans and Non-Vegetarians in the Adventist Health Study-2 Cohort.
The Biology of Veganism: Plasma Metabolomics Analysis Reveals Distinct Profiles of Vegans and Non-Vegetarians in the Adventist Health Study-2 Cohort.
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DOI：
10.3390/nu14030709
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发表时间：
2022-02-08
期刊：
影响因子：
5.9
通讯作者：
Fraser GE
中科院分区：
文献类型：
--
作者：
Miles FL;Orlich MJ;Mashchak A;Chandler PD;Lampe JW;Duerksen-Hughes P;Fraser GE
目前尚不清楚素食饮食模式如何影响参与调节慢性疾病的生物过程的血浆代谢物。我们试图通过使用超高效液相色谱质谱（UPLC-MS/MS）的全球代谢组学分析来识别复临健康研究-2队列中素食者（不吃肉、鸡蛋、乳制品）和非素食者（食用≥28 g/天红肉）的血浆代谢谱。使用线性回归模型分析代谢物或生化亚类的丰度差异，调整替代变量和混淆变量，并通过交叉验证来模拟独立样本的结果。随机森林被用作分类的学习工具，主成分分析被用于识别相关代谢物簇。校正错误发现后，超过60%的代谢物（586/930）存在协变量调整后的代谢物丰度差异。绝大多数差异丰度代谢物或代谢物亚类在素食者中丰度较低，包括黄嘌呤、组氨酸、支链脂肪酸、乙酰化肽、神经酰胺和长链酰基肉碱等。许多这些代谢物亚类在胰岛素失调、心脏代谢表型和炎症中起作用。对纯素食者和非素食者代谢谱的分析显示，这两种饮食组存在巨大差异，反映了动物和植物产品消费的差异。这些代谢物作为食物摄入的生物标志物，许多对心脏代谢疾病具有潜在的病理生理后果。
It is unclear how vegetarian dietary patterns influence plasma metabolites involved in biological processes regulating chronic diseases. We sought to identify plasma metabolic profiles distinguishing vegans (avoiding meat, eggs, dairy) from non-vegetarians (consuming ≥28 g/day red meat) of the Adventist Health Study-2 cohort using global metabolomics profiling with ultra-performance liquid chromatography mass spectrometry (UPLC-MS/MS). Differences in abundance of metabolites or biochemical subclasses were analyzed using linear regression models, adjusting for surrogate and confounding variables, with cross-validation to simulate results from an independent sample. Random forest was used as a learning tool for classification, and principal component analysis was used to identify clusters of related metabolites. Differences in covariate-adjusted metabolite abundance were identified in over 60% of metabolites (586/930), after adjustment for false discovery. The vast majority of differentially abundant metabolites or metabolite subclasses showed lower abundance in vegans, including xanthine, histidine, branched fatty acids, acetylated peptides, ceramides, and long-chain acylcarnitines, among others. Many of these metabolite subclasses have roles in insulin dysregulation, cardiometabolic phenotypes, and inflammation. Analysis of metabolic profiles in vegans and non-vegetarians revealed vast differences in these two dietary groups, reflecting differences in consumption of animal and plant products. These metabolites serve as biomarkers of food intake, many with potential pathophysiological consequences for cardiometabolic diseases.
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影响因子：
7.7
作者：
Floegel A;Stefan N;Yu Z;Mühlenbruch K;Drogan D;Joost HG;Fritsche A;Häring HU;Hrabě de Angelis M;Peters A;Roden M;Prehn C;Wang-Sattler R;Illig T;Schulze MB;Adamski J;Boeing H;Pischon T
通讯作者：
Pischon T
DOI：
10.3233/jad-2011-111202
发表时间：
2012
期刊：
Journal of Alzheimer's disease : JAD
影响因子：
--
作者：
Filippov V;Song MA;Zhang K;Vinters HV;Tung S;Kirsch WM;Yang J;Duerksen-Hughes PJ
通讯作者：
Duerksen-Hughes PJ
影响因子：
13.6
作者：
Aronov, Pavel A.;Luo, Frank J. -G.;Meyer, Timothy W.
通讯作者：
Meyer, Timothy W.
影响因子：
5.4
作者：
Ding Y;Svingen GF;Pedersen ER;Gregory JF;Ueland PM;Tell GS;Nygård OK
通讯作者：
Nygård OK
影响因子：
7.1
作者：
Chandler, Paulette D.;Balasubramanian, Raji;Rexrode, Kathryn M.
通讯作者：
Rexrode, Kathryn M.