Do neutrophils play a role in establishing liver abscesses and distant metastases caused by Klebsiella pneumoniae?
Do neutrophils play a role in establishing liver abscesses and distant metastases caused by Klebsiella pneumoniae?
复制标题
DOI：
10.1371/journal.pone.0015005
复制
发表时间：
2010-11-30
期刊：
影响因子：
3.7
通讯作者：
Siu LK
中科院分区：
文献类型：
--
作者：
Lin JC;Chang FY;Fung CP;Yeh KM;Chen CT;Tsai YK;Siu LK
血清型K1肺炎克雷伯菌是肝脓肿和眼内炎的主要病因。本研究旨在确定中性粒细胞在由血清型K1肺炎克雷伯菌引起的远处转移性并发症发展中的作用。体外细胞模型用于评估血清耐药性和中性粒细胞介导的杀伤。给BALB/c小鼠注射含有被吞噬肺炎克雷伯菌的中性粒细胞。血清型K1肺炎克雷伯菌对血清杀伤、中性粒细胞介导的吞噬和细胞内杀伤的抗性显著高于非K1分离株（p<0.01）。电镜检查结果与生物测定结果相似。腹腔注射含有吞噬血清型K1肺炎克雷伯菌的中性粒细胞导致皮下组织、肺和肝脏等多个部位形成脓肿，而在注射非K1分离物的小鼠中未观察到脓肿形成。血清型K1肺炎克雷伯菌对补体和中性粒细胞介导的细胞内杀伤的耐药性导致肺炎克雷伯菌通过血液传播。逃避嗜中性粒细胞细胞内杀伤可能有助于远处转移的传播和建立。因此，中性粒细胞扮演了帮助肺炎克雷伯菌和促进建立肝脓肿和远处转移并发症的载体的角色。
Serotype K1 Klebsiella pneumoniae is a major cause of liver abscesses and endophthalmitis. This study was designed to identify the role of neutrophils in the development of distant metastatic complications that were caused by serotype K1 K. pneumoniae. An in vitro cellular model was used to assess serum resistance and neutrophil-mediated killing. BALB/c mice were injected with neutrophils containing phagocytosed K. pneumoniae. Serotype K1 K. pneumoniae was significantly more resistant to serum killing, neutrophil-mediated phagocytosis and intra-cellular killing than non-K1 isolates (p<0.01). Electron microscopic examination had similar findings as in the bioassay findings. Intraperitoneal injection of neutrophils containing phagocytosed serotype K1 K. pneumoniae led to abscess formation in multiple sites including the subcutaneous tissue, lung, and liver, whereas no abscess formation was observed in mice injected with non-K1 isolates. The resistance of serotype K1 K. pneumoniae to complement- and neutrophil-mediated intracellular killing results in the dissemination of K. pneumoniae via the bloodstream. Escape from neutrophil intracellular killing may contribute to the dissemination and establishment of distant metastases. Thus, neutrophils play a role as a vehicle for helping K. pneumoniae and contributing to the establishment of liver abscess and distant metastatic complications.
登录
查看更多内容
影响因子：
--
作者：
Itou, Takuya;Collins, L. Vincent;Karlsson, Anna
通讯作者：
Karlsson, Anna
影响因子：
9.4
作者：
CRYZ, SJ;MORTIMER, PM;GERMANIER, R
通讯作者：
GERMANIER, R
影响因子：
4.5
作者：
HIGHSMITH, AK;JARVIS, WR
通讯作者：
JARVIS, WR
鼠伤寒沙门氏菌持续存在于慢性感染 Nramp1+/+ 小鼠肠系膜淋巴结的巨噬细胞内，并可通过 IFNγ 中和重新激活。
影响因子：
15.3
作者：
Monack, DM;Bouley, DM;Falkow, S
通讯作者：
Falkow, S
影响因子：
158.5
作者：
MOWAT, AG;BAUM, J
通讯作者：
BAUM, J