Loss of muscular force in isolated rat diaphragms is related to changes in muscle fibre size
Loss of muscular force in isolated rat diaphragms is related to changes in muscle fibre size
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离体大鼠膈肌肌力的丧失与肌纤维尺寸的变化有关
DOI：
10.1088/1361-6579/abdf3a
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发表时间：
2021
影响因子：
3.2
通讯作者：
Schumann S
中科院分区：
文献类型：
--
作者：
Lieb S;Schumann S
目的长时间机械通气时，横隔肌的被动可导致机械通气所致的横隔肌功能障碍，其原因是横隔肌肌力下降。电刺激可以用来调节横隔肌的力量。因此，我们打算研究基于电脉冲刺激的横隔肌力量。方法将Wistar大鼠的横隔肌切开，包埋在不同的孵育液中，放置在横隔力测量装置中。测定隔膜产生的压力幅度与包埋液、刺激频率和时间(360min)的关系。主要结果是，体外培养的横隔膜在高钾浓度的孵育液中不会产生压力，如果在细胞外钾浓度的孵育液中植入，则最高可达>；20cmH2O。虽然培养360min后仍能保持较好的活力(78%)，但240min后膜力下降了90.8%。如果每隔20分钟进行一次刺激，与每120分钟进行一次刺激相比，隔膜力量的下降进行得更快。每120min刺激一次，I型肌纤维最大。电刺激后，2b/x型肌细胞在横隔膜中的纤维直径小于未刺激的横隔膜。意义：在体外条件下，横隔膜的力量取决于孵育液和激活条件。与活动相关的横隔膜肌力变化伴随着肌肉纤维大小的特定变化。
ObjectivePassivity of the diaphragm during prolonged mechanical ventilation can lead to ventilation-induced diaphragmatic dysfunction reasoned by a reduction of diaphragmatic muscle strength. Electrical stimulation may be utilised to modulate diaphragm muscle strength. Therefore we intended to investigate diaphragmatic muscle strength based on stimulation with electric impulses.ApproachDiaphragms of Wistar rats were excised, embedded in various incubation solutions and placed in a diaphragm force measurement device. Pressure amplitudes generated by the diaphragm in dependency of the embedding solution, stimulation frequency and time (360 min) were determined. Furthermore, the diaphragms were histologically evaluated.Main resultsThe ex vivo diaphragms evoked no pressure if embedded in incubation solutions with high potassium concentrations and up to> 20 cmH 2 O if embedded in incubation solutions with extracellular potassium concentrations. Although vitality was well maintained after 360 min (78%) cultivation, the diaphragm's force dropped by 90.8% after 240 min. The decline in the diaphragm's force progressed faster if stimulation was performed every 20 min compared to every 120 min. The size of Type I muscle fibres was largest in diaphragms stimulated every 120 min. The fibre size of Type 2b/x muscle cells was lower in diaphragms after electrical stimulation compared to non-stimulated diaphragms.SignificanceThe force that the diaphragm can develop in ex vivo conditions depends on the incubation solution and the conditions of activation. Activity-related changes in the diaphragm's muscular force are accompanied by specific changes in muscle fibre size.
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影响因子：
3.3
作者：
S. Powers;D. Criswell;R. A. Herb;H. Demirel;S. Dodd
通讯作者：
S. Dodd
运输中的肾脏保存：II. 12 小时储存和航空运输的简单技术1
DOI：
--
发表时间：
1969
期刊：
影响因子：
--
作者：
G. Collins;M. Bravo;P. Terasaki
通讯作者：
P. Terasaki
离体大鼠隔膜体外模型中的机械刺激、电刺激和力测量
影响因子：
3.2
作者：
C. Armbruster;C. Dassow;K. Gamerdinger;Matthias Schneider;M. Šumkauskaitė;J. Guttmann;S. Schumann
通讯作者：
S. Schumann
海藻糖对大鼠肌瓣的防腐作用
影响因子：
1.5
作者：
C. W. Zhan;Yoshihisa Suzuki;A. Kitahara;H. Wada;Y. Nishimura
通讯作者：
Y. Nishimura
DOI：
10.1186/cc9094
发表时间：
2010
期刊：
Critical care (London, England)
影响因子：
--
作者：
Hermans G;Agten A;Testelmans D;Decramer M;Gayan-Ramirez G
通讯作者：
Gayan-Ramirez G