Changes in cytokine levels and NK cell activation associated with influenza.
Changes in cytokine levels and NK cell activation associated with influenza.
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DOI：
10.1371/journal.pone.0025060
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Altfeld M
中科院分区：
文献类型：
--
作者：
Jost S;Quillay H;Reardon J;Peterson E;Simmons RP;Parry BA;Bryant NN;Binder WD;Altfeld M
几项研究强调了鼠自然杀伤（NK）细胞在控制流感感染中的重要作用。然而，人类NK细胞在急性流感感染，包括2009年大流行性H1N1流感病毒感染中的反应记录甚少。在这里，我们研究了与流感感染和疫苗接种相关的NK细胞表型和功能以及血浆细胞因子水平的变化。我们发现，外周血NK细胞的绝对数量，特别是那些CD 56 bright NK细胞，急性流感感染后下降，而这个NK细胞亚群扩大肌内流感疫苗接种后。暴露于流感抗原的NK细胞被激活，在感染季节性流感病毒株的研究受试者中，表达CD 69的NK细胞比例更高。疫苗接种导致CD 25 + NK细胞水平增加，尤其是CD 56 + CD 25 + NK细胞，而流感感染个体的外周血中存在该亚群的量减少，并且主要在感染2009年大流行性H1N1流感病毒的研究受试者中。最后，急性流感感染与低血浆浓度的炎性细胞因子（包括IFN-γ、MIP-1β、IL-2和IL-15）和高水平的抗炎细胞因子IL-10和IL-1 ra相关。总而言之，这些数据表明CD 56 bright NK细胞亚群在对流感的应答中的作用，可能涉及它们向感染组织的募集以及局部产生和/或摄取炎性细胞因子。
Several studies have highlighted the important role played by murine natural killer (NK) cells in the control of influenza infection. However, human NK cell responses in acute influenza infection, including infection with the 2009 pandemic H1N1 influenza virus, are poorly documented. Here, we examined changes in NK cell phenotype and function and plasma cytokine levels associated with influenza infection and vaccination. We show that absolute numbers of peripheral blood NK cells, and particularly those of CD56bright NK cells, decreased upon acute influenza infection while this NK cell subset expanded following intramuscular influenza vaccination. NK cells exposed to influenza antigens were activated, with higher proportions of NK cells expressing CD69 in study subjects infected with seasonal influenza strains. Vaccination led to increased levels of CD25+ NK cells, and notably CD56bright CD25+ NK cells, whereas decreased amounts of this subset were present in the peripheral blood of influenza infected individuals, and predominantly in study subjects infected with the 2009 pandemic H1N1 influenza virus. Finally, acute influenza infection was associated with low plasma concentrations of inflammatory cytokines, including IFN-γ, MIP-1β, IL-2 and IL-15, and high levels of the anti-inflammatory cytokines IL-10 and IL-1ra. Altogether, these data suggest a role for the CD56bright NK cell subset in the response to influenza, potentially involving their recruitment to infected tissues and a local production and/or uptake of inflammatory cytokines.
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