Synovial mesenchymal stem cells promote meniscus regeneration augmented by an autologous Achilles tendon graft in a rat partial meniscus defect model.
Synovial mesenchymal stem cells promote meniscus regeneration augmented by an autologous Achilles tendon graft in a rat partial meniscus defect model.
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DOI：
10.1002/stem.2030
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发表时间：
2015-06
期刊：
影响因子：
5.2
通讯作者：
Sekiya, Ichiro
中科院分区：
文献类型：
--
作者：
Ozeki, Nobutake;Muneta, Takeshi;Matsuta, Seiya;Koga, Hideyuki;Nakagawa, Yusuke;Mizuno, Mitsuru;Tsuji, Kunikazu;Mabuchi, Yo;Akazawa, Chihiro;Kobayashi, Eiji;Saito, Tomoyuki;Sekiya, Ichiro
尽管半月板缺陷和退变与骨性关节炎的后期发展密切相关，但再生医学策略的前景是预防和/或延缓疾病的进展。肌腱移植和骨髓间充质干细胞(MSCs)移植重建半月板的动物模型已被证明；然而，这些手术在临床研究中并未显示出同样的效果。在此，我们的目的是研究肌腱移植物经外源性滑膜来源的MSCs处理后预防大鼠部分半月板损伤模型软骨退变的能力。我们切除了内侧半月板的前半部分，并移植了自体跟腱，无论是否用滑膜MSCs对肌腱进行了10分钟的预处理。分别于术后2、4、8周对半月板及周围软骨进行评估(n=5)。肌腱移植增加了半月板大小，与滑膜MSCs无关。术后4周和8周，肌腱+MSC组再生半月板的组织学评分明显好于其他两组。术后8周，肌腱+间充质干细胞组关节软骨的大体和组织学评分均明显好于对照组。通过荧光素酶+、LacZ+、DII+和/或GFP+滑膜MSCs和/或GFP+肌腱的细胞跟踪分析，移植的滑膜MSCs在移植肌腱和自然半月板结合部位周围存活。流式细胞仪分析显示，移植的滑膜MSCs在7d后仍保持其MSC特性，宿主滑膜组织中也含有具有MSC特性的细胞。滑膜间充质干细胞促进自体跟腱移植增强的半月板再生，防止大鼠软骨退变。干细胞2015；33：1927-1938
Although meniscus defects and degeneration are strongly correlated with the later development of osteoarthritis, the promise of regenerative medicine strategies is to prevent and/or delay the disease's progression. Meniscal reconstruction has been shown in animal models with tendon grafting and transplantation of mesenchymal stem cells (MSCs); however, these procedures have not shown the same efficacy in clinical studies. Here, our aim was to investigate the ability of tendon grafts pretreated with exogenous synovial-derived MSCs to prevent cartilage degeneration in a rat partial meniscus defect model. We removed the anterior half of the medial meniscus and grafted autologous Achilles tendons with or without a 10-minute pretreatment of the tendon with synovial MSCs. The meniscus and surrounding cartilage were evaluated at 2, 4, and 8 weeks (n = 5). Tendon grafts increased meniscus size irrespective of synovial MSCs. Histological scores for regenerated menisci were better in the tendon + MSC group than in the other two groups at 4 and 8 weeks. Both macroscopic and histological scores for articular cartilage were significantly better in the tendon + MSC group at 8 weeks. Implanted synovial MSCs survived around the grafted tendon and native meniscus integration site by cell tracking assays with luciferase+, LacZ+, DiI+, and/or GFP+ synovial MSCs and/or GFP+ tendons. Flow cytometric analysis showed that transplanted synovial MSCs retained their MSC properties at 7 days and host synovial tissue also contained cells with MSC characteristics. Synovial MSCs promoted meniscus regeneration augmented by autologous Achilles tendon grafts and prevented cartilage degeneration in rats. Stem Cells 2015;33:1927–1938
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影响因子：
4.2
作者：
Matsukura Y;Muneta T;Tsuji K;Koga H;Sekiya I
通讯作者：
Sekiya I
影响因子：
5.2
作者：
Koga, Hideyuki;Muneta, Takeshi;Sekiya, Ichiro
通讯作者：
Sekiya, Ichiro
影响因子：
2.8
作者：
Kessler, Michael W.;Barr, Jerome;Chahine, Nadeen O.
通讯作者：
Chahine, Nadeen O.
DOI：
10.1302/0301-620x.74b6.1447257
发表时间：
1992-11-01
影响因子：
--
作者：
KOHN, D;WIRTH, CJ;WULKER, N
通讯作者：
WULKER, N
影响因子：
4
作者：
Caplan, Arnold I.;Dennis, James E.
通讯作者：
Dennis, James E.