Transcription factor PyHY5 binds to the promoters of PyWD40 and PyMYB10 and regulates its expression in red pear 'Yunhongli No. 1'.
Transcription factor PyHY5 binds to the promoters of PyWD40 and PyMYB10 and regulates its expression in red pear 'Yunhongli No. 1'.
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转录因子PyHY5与PyWD40和PyMYB10的启动子结合并调节其在红梨‘云红梨1号’中的表达。
DOI：
10.1016/j.plaphy.2020.07.008
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发表时间：
2020-07
影响因子：
6.5
通讯作者：
Su Jun
中科院分区：
文献类型：
--
作者：
Wang Yuying;Zhang Xiaodong;Zhao Yiran;Yang Jin;He Yingyun;Li Guochang;Ma Weirong;Huang Xinglong;Su Jun
“云红梨1号”是一种珍贵的红梨种质资源，因其色泽鲜红、品质优良而受到市场的青睐。光诱导运输因子基因MYB 10、WD 40和HY 5的表达，进而激活花青素苷生物合成途径中的关键基因的表达，促进花青素苷的合成和积累，从而赋予红色。蛋白HY 5被认为是诱导花青素苷生物合成的关键调节因子。MYB 10基因与HY 5通过与G-box基序的结合，在拟南芥、苹果和梨中物理地相互作用以正向调节花青素苷的生物合成。然而，这些转录因子是如何被阳光调控的在‘云红粒1号’中仍不清楚。本研究克隆了转录因子PyHY 5，亚细胞定位实验表明PyHY 5定位于细胞核内。PyHY 5的DNA片段具有bZIP家族的典型BRLZ结构域，然后与PyMYB 10和PyWD 40的启动子序列进行比对。电泳迁移率变动和瞬时表达实验表明，PyHY 5能够直接识别PyMYB 10和PyWD 40启动子中的G-box基序，并与之结合，从而通过共表达促进转录激活。结果表明，PyHY 5与PyMYB 10和PyWD 40启动子的G-box基序结合，增强了其表达，进而促进了“云红丽1号”花色苷的积累。
‘Yunhongli No. 1’ is a rare and well-colored red pear (Pyrus pyrifolia) germplasm resource, and is popular in the market due to its bright red color and high quality. Light induces the expression of transportation factor genesMYB10,WD40, andHY5, which then activate the expression of critical genes in the anthocyanin biosynthesis pathway to promote the synthesis and accumulation of anthocyanin, thus giving the red coloration. Protein HY5 is considered to be a key regulator for induction of anthocyanin biosynthesis. TheMYB10genes physically interact with HY5 to positively regulate anthocyanin biosynthesis in Arabidopsis, apple, and pear by binding to G-box motifs. However, how these transcription factors are regulated by sunlight remains unclear in ‘Yunhongli No. 1’. In this study, the transcription factor PyHY5 was cloned, and subcellular localization assay showed that PyHY5 was distributed in the nucleus. The DNA fragments ofPyHY5had a typical BRLZ domain of the bZIP family, and then were aligned against the promoter sequences ofPyMYB10andPyWD40. Electrophoretic mobility shift and transient expression assays showed that PyHY5 could directly recognize and bind to the G-box motifs in the promoters ofPyMYB10andPyWD40, and so boosted transcriptional activation by co-expression. The results demonstrated that PyHY5 binding to G-box motifs of the promoters ofPyMYB10andPyWD40, enhanced its expression, and then promoted accumulation of anthocyanin in red ‘Yunhongli No. 1’.
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DOI：
10.21273/jashs.129.1.0006
发表时间：
2004
影响因子：
1.9
作者：
W. Steyn;D. Holcroft;S. Wand;G. Jacobs
通讯作者：
W. Steyn;D. Holcroft;S. Wand;G. Jacobs
DOI：
10.1111/j.1365-313x.2006.02964.x
发表时间：
2007-02
期刊：
The Plant journal : for cell and molecular biology
影响因子：
--
作者：
Espley RV;Hellens RP;Putterill J;Stevenson DE;Kutty-Amma S;Allan AC
通讯作者：
Allan AC
转录组分析揭示了与“Red Bartlett”（Pyrus communis L.）褪色相关的候选基因。
影响因子：
5.6
作者：
Wang Z;Du H;Zhai R;Song L;Ma F;Xu L
通讯作者：
Xu L
影响因子：
7.2
作者：
Yao, Gaifang;Ming, Meiling;Wu, Jun
通讯作者：
Wu, Jun
蓝光信号转导通路参与“红早酥”梨花青素积累
影响因子：
4.3
作者：
Tao, Ruiyan;Bai, Songling;Teng, Yuanwen
通讯作者：
Teng, Yuanwen