Remodeling of extracellular matrix in the urinary bladder of paraplegic rats results in increased compliance and delayed fiber recruitment 16 weeks after spinal cord injury.
Remodeling of extracellular matrix in the urinary bladder of paraplegic rats results in increased compliance and delayed fiber recruitment 16 weeks after spinal cord injury.
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DOI：
10.1016/j.actbio.2022.01.015
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发表时间：
2022-03-15
影响因子：
9.7
通讯作者：
Roccabianca S
中科院分区：
文献类型：
--
作者：
Tuttle TG;Lujan HL;Tykocki NR;DiCarlo SE;Roccabianca S
膀胱在储存尿液的同时保持低膀胱内压力的能力是确保器官正常功能的关键，并强调了组织力学在下尿道中发挥的关键作用。脊髓损伤后失去与膀胱的棘上神经元连接可导致膀胱壁结构的重塑，这可能改变其力学特性。在这项研究中，我们研究了大鼠脊髓损伤后16周膀胱细胞外基质的形态和力学特性是否与接受假手术的动物相比发生了改变。我们使用组织学分析、拉伸测试和本构建模来测量和量化膀胱几何形状和力学行为的变化。我们的研究结果表明，损伤后16周的截瘫动物膀胱顺应性增加。此外，本构模型显示，膨胀性的增加是由胶原纤维波浪度的增加驱动的，这改变了加载过程中纤维招募的分布。膀胱在低压下储存尿液的能力是确保器官正常功能的关键。这突出了力学在下尿道中发挥的重要作用。脊髓损伤引起的膀胱神经连接失去控制可导致膀胱壁结构的改变，从而改变机械特性。我们发现脊髓损伤后16周的大鼠膀胱壁微结构比健康动物更具有顺应性。这很重要，因为这是迄今为止分析的受伤后最长的时间。了解膀胱在病理条件下的极端重塑能力是告知新的可能治疗方法的关键。
The ability of the urinary bladder to maintain low intravesical pressures while storing urine is key in ensuring proper organ function and highlights the key role that tissue mechanics plays in the lower urinary tract. Loss of supraspinal neuronal connections to the bladder after spinal cord injury can lead to remodeling of the structure of the bladder wall, which may alter its mechanical characteristics. In this study, we investigate if the morphology and mechanical properties of the bladder extracellular matrix are altered in rats 16 weeks after spinal cord injury as compared to animals who underwent sham surgery. We measured and quantified the changes in bladder geometry and mechanical behavior using histological analysis, tensile testing, and constitutive modeling. Our results suggest bladder compliance is increased in paraplegic animals 16 weeks post-injury. Furthermore, constitutive modeling showed that increased distensibility was driven by an increase in collagen fiber waviness, which altered the distribution of fiber recruitment during loading. The ability of the urinary bladder to store urine under low pressure is key in ensuring proper organ function. This highlights the important role that mechanics plays in the lower urinary tract. Loss of control of neurologic connection to the bladder from spinal cord injury can lead to changes of the structure of the bladder wall, resulting in altered mechanical characteristics. We found that the bladder wall’s microstructure in rats 16 weeks after spinal cord injury is more compliant than in healthy animals. This is significant since it is the longest time post-injury analyzed, to date. Understanding the extreme remodeling capabilities of the bladder in pathological conditions is key to inform new possible therapies.
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影响因子：
2.2
作者：
Giannantoni, A;Di Stasi, SM;Vespasiani, G
通讯作者：
Vespasiani, G
影响因子：
3.5
作者：
Cheng F;Birder LA;Kullmann FA;Hornsby J;Watton PN;Watkins S;Thompson M;Robertson AM
通讯作者：
Robertson AM
影响因子：
6.6
作者：
Gloeckner, DC;Sacks, MS;Chancellor, MB
通讯作者：
Chancellor, MB
影响因子：
2.4
作者：
Chen, Joseph;Drzewiecki, Beth A.;Pope, John C.
通讯作者：
Pope, John C.
影响因子：
3.4
作者：
Chen, Huan;Liu, Yi;Kassab, Ghassan S.
通讯作者：
Kassab, Ghassan S.