Immunolocalisation studies on six matrix metalloproteinases and their inhibitors, TIMP-1 and TIMP-2, in synovia from patients with osteo- and rheumatoid arthritis.
Immunolocalisation studies on six matrix metalloproteinases and their inhibitors, TIMP-1 and TIMP-2, in synovia from patients with osteo- and rheumatoid arthritis.
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对骨关节炎和类风湿性关节炎患者滑液中六种基质金属蛋白酶及其抑制剂 TIMP-1 和 TIMP-2 的免疫定位研究。
DOI：
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发表时间：
1995
影响因子：
27.4
通讯作者：
D. Hamblen
中科院分区：
文献类型：
--
作者：
R. Hembry;M. Bagga;J. Reynolds;D. Hamblen
目的：评估基质金属蛋白酶（MMPs）及其抑制剂（TIMPs）在关节炎过程中的可能重要性。方法：用间接免疫荧光显微镜分析了7个类风湿性关节炎关节和3个骨关节炎关节的滑膜样本。使用特异性人抗血清，我们记录了胶原酶、基质溶解素1和2、基质溶解素、明胶酶A和B、TIMP-1和TIMP-2的频率和分布。结果：溶基质素1存在于所有滑膜中，与细胞外基质结合，在细胞内，或两者结合，表明溶基质素合成。基质溶解素只存在于一个活跃的炎症滑膜，胶原酶和明胶酶A的局部合成被认为是在四个滑膜。基质分解素2和TIMP-2未见表达，但TIMP-1在5个滑膜中有表达，在2个活动性滑膜中TIMP-1阳性细胞的分布较MMP广泛。结论--所有滑膜中基质溶解素1的存在明确地暗示了这种酶在关节损伤中的作用。胶原酶、明胶酶A和基质溶解素也可能在类风湿性关节炎中起作用，但在骨关节炎中不显著。然而，在这些MMPs的合成中发现了显着的区域差异，这不仅表明这些疾病是间歇性的，而且对酶合成的控制是焦点。只有TIMP-1可以被认为是一种抑制因子。
OBJECTIVE--To assess the likely importance of matrix metalloproteinases (MMPs) and their inhibitors (TIMPs) in the arthritic process. METHODS--Synovial samples from seven joints with rheumatoid arthritis and three osteoarthritic joints were analysed by indirect immunofluorescence microscopy. Using specific human antisera, we documented the frequencies and distributions of collagenase, stromelysins 1 and 2, matrilysin, gelatinases A and B, TIMP-1, and TIMP-2. RESULTS--Stromelysin 1 was found in all synovia, bound to extracellular matrix, within cells, or both, indicating stromelysin synthesis. Matrilysin was present in only one active inflammatory synovium, and focal synthesis of collagenase and gelatinase A was seen in four synovia. Stromelysin 2 and TIMP-2 were not observed, but TIMP-1 synthesis was seen in five synovia, and in two active synovia the distribution of TIMP-1 positive cells was more widespread than that of MMPs. CONCLUSIONS--The presence of stromelysin 1 in all synovia clearly implicates this enzyme in joint damage. Collagenase, gelatinase A and matrilysin may also have a role in rheumatoid arthritis, but are not significant in osteoarthritis. However, marked regional variations were found in the synthesis of these MMPs, indicating not only that these diseases are episodic but that control of enzyme synthesis is focal. Only TIMP-1 may be considered an inhibitory factor.
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影响因子：
27.4
作者：
OKADA, Y;TAKEUCHI, N;NAGASE, H
通讯作者：
NAGASE, H
人溶基质素和溶基质素 2 基因的克隆：类风湿滑膜成纤维细胞中的差异表达。
影响因子：
2.9
作者：
Sirum,KL;Brinckerhoff,CE
通讯作者：
Brinckerhoff,CE
DOI：
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发表时间：
1992
期刊：
Laboratory investigation; a journal of technical methods and pathology
影响因子：
--
作者：
Y. Okada;M. Shinmei;O. Tanaka;K. Naka;A. Kimura;Isao Nakanishi;M. Bayliss;K. Iwata;H. Nagase
通讯作者：
Y. Okada;M. Shinmei;O. Tanaka;K. Naka;A. Kimura;Isao Nakanishi;M. Bayliss;K. Iwata;H. Nagase
影响因子：
--
作者：
GRAVALLESE, EM;DARLING, JM;GLIMCHER, LH
通讯作者：
GLIMCHER, LH
金属蛋白酶和金属蛋白酶抑制剂在人关节炎滑膜中的表达。
影响因子：
--
作者：
McCachren,SS
通讯作者：
McCachren,SS