High glucose aggravates the detrimental effects of pancreatic stellate cells on Beta-cell function.
High glucose aggravates the detrimental effects of pancreatic stellate cells on Beta-cell function.
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高血糖会加剧胰腺星状细胞对 β 细胞功能的不利影响
DOI：
10.1155/2014/165612
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发表时间：
2014
影响因子：
2.8
通讯作者：
Sun Z
中科院分区：
文献类型：
--
作者：
Zha M;Xu W;Zhai Q;Li F;Chen B;Sun Z
背景和目的。我们在体内和体外评估了PSCs对β-细胞功能和凋亡的影响。材料与方法。将PSCs移植到Wistar和Goto-Kakizaki （GK）大鼠体内。移植16周后，评估β细胞功能、凋亡和胰岛纤维化。在体外，通过测量胰岛素分泌、INS-1细胞存活、凋亡和内质网应激（ER应激）相关CHOP表达，评估PSCs条件培养基（PSCs- cm）和/或高浓度葡萄糖对INS-1细胞功能的影响。结果。PSCs移植加重了GK大鼠β-细胞功能受损，但对Wistar大鼠无明显影响。在体外，PSCs-CM导致INS-1细胞活力和胰岛素分泌受损，细胞凋亡增加，在高糖环境下更为明显。结论。我们的研究表明，PSCs在体外和体内诱导β细胞衰竭。
Background and Aims. We here assess the effects of PSCs on β-cell function and apoptosis in vivo and in vitro. Materials and Methods. PSCs were transplanted into Wistar and Goto-Kakizaki (GK) rats. Sixteen weeks after transplantation, β-cell function, apoptosis, and islet fibrosis were assessed. In vitro the effects of PSCs conditioned medium (PSCs-CM) and/or high concentration of glucose on INS-1 cell function was assessed by measuring insulin secretion, INS-1 cell survival, apoptosis, and endoplasmic reticulum stress (ER stress) associated CHOP expression. Results. PSCs transplantation exacerbated the impaired β-cell function in GK rats, but had no significant effects in Wistar rats. In vitro, PSCs-CM caused impaired INS-1 cell viability and insulin secretion and increased apoptosis, which were more pronounced in the presence of high glucose. Conclusion. Our study demonstrates that PSCs induce β-cell failure in vitro and in vivo.
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影响因子：
4
作者：
Hong, Oak-Kee;Lee, Seung-Hwan;Yoon, Kun-Ho
通讯作者：
Yoon, Kun-Ho
DOI：
10.1016/j.bbrc.2011.09.087
发表时间：
2011-10-22
影响因子：
3.1
作者：
Lee, Esder;Ryu, Gyeong Ryul;Song, Ki-Ho
通讯作者：
Song, Ki-Ho
影响因子：
4
作者：
Apte MV;Pirola RC;Wilson JS
通讯作者：
Wilson JS
影响因子：
29.4
作者：
Bachem, MG;Schneider, E;Adler, G
通讯作者：
Adler, G
影响因子：
24.5
作者：
Apte, MV;Haber, PS;Wilson, JS
通讯作者：
Wilson, JS