Heparin blocks transfer of extracellular vesicles between donor and recipient cells.
Heparin blocks transfer of extracellular vesicles between donor and recipient cells.
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DOI：
10.1007/s11060-013-1235-y
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发表时间：
2013-12
影响因子：
3.9
通讯作者：
Maguire, Casey A.
中科院分区：
文献类型：
--
作者：
Atai, Nadia A.;Balaj, Leonora;van Veen, Henk;Breakefield, Xandra O.;Jarzyna, Peter A.;Van Noorden, Cornelis J. F.;Skog, Johan;Maguire, Casey A.
细胞外囊泡（EV）与肿瘤发生有关。可以阻断EV结合和摄取到受体细胞中的生物分子可能具有治疗价值，以及增强对EV生物学的理解。在这里，我们表明肝素与肿瘤衍生的以及非肿瘤衍生的EV进入受体细胞的摄取相互作用。用肝素孵育胶质瘤细胞衍生的EV导致通过透射电子显微镜观察到的微米级结构，EV在这些结构内清晰可见。肝素的加入大大减少了标记的EV从供体转移到受体肿瘤细胞。我们还显示了肝素和EV之间的直接相互作用，使用共聚焦显微镜。我们发现EV摄取的阻断是在细胞结合的水平，而不是内化。最后，含有EGFRvIII mRNA的神经胶质瘤衍生EV与肝素的孵育减少了该信息向受体细胞的转移。肝素对EV摄取的影响可能为研究EV功能提供独特的工具。它还可以促进肝素或其衍生物作为EV发挥作用的疾病的治疗方法的研究。
Extracellular vesicles (EVs) have been implicated in tumorigenesis. Biomolecules which can block EV binding and uptake into recipient cells may be of therapeutic value as well as enhance understanding of EV biology. Here, we show that heparin interacts with uptake of tumor-derived as well as non-tumor-derived EVs into recipient cells. Incubation of glioma cell-derived EVs with heparin resulted in micron-sized structures observed by transmission electron microscopy, with EVs clearly visible within these structures. Inclusion of heparin greatly diminished transfer of labeled EVs from donor to recipient tumor cells. We also show a direct interaction between heparin and EVs using confocal microscopy. We found that the block in EV uptake was at the level of cell binding and not internalization. Finally, incubation of glioma-derived EVs containing EGFRvIII mRNA with heparin reduced transfer of this message to recipient cells. The effect of heparin on EVs uptake may provide a unique tool to study EV function. It may also foster research of heparin or its derivatives as a therapeutic for disease in which EVs play a role.
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DOI：
10.1590/s0100-879x1999000500005
发表时间：
1999-05-01
影响因子：
2.3
作者：
Nader, HB;Chavante, SF;Dietrich, CP
通讯作者：
Dietrich, CP
影响因子：
15.9
作者：
Dolecek, Therese A.;Propp, Jennifer M.;Kruchko, Carol
通讯作者：
Kruchko, Carol
影响因子：
4.8
作者：
Nagai, A;Sato, T;Sumida, M
通讯作者：
Sumida, M
影响因子：
12.4
作者：
Maguire, Casey A.;Balaj, Leonora;Skog, Johan
通讯作者：
Skog, Johan
影响因子：
7.5
作者：
Van Noorden, Cornelis J. F.;van Sluis, Geerte L.;Spek, C. Arnold
通讯作者：
Spek, C. Arnold