Dietary fatty acid intake and gut microbiota determine circulating endocannabinoidome signaling beyond the effect of body fat.
Dietary fatty acid intake and gut microbiota determine circulating endocannabinoidome signaling beyond the effect of body fat.
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饮食脂肪酸的摄入和肠道菌群确定循环内源性大麻素的信号传导超出了体内脂肪的作用。
DOI：
10.1038/s41598-020-72861-3
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发表时间：
2020-09-29
影响因子：
4.6
通讯作者：
Veilleux A
中科院分区：
文献类型：
--
作者：
Castonguay-Paradis S;Lacroix S;Rochefort G;Parent L;Perron J;Martin C;Lamarche B;Raymond F;Flamand N;Di Marzo V;Veilleux A
内源性大麻素包括几种由脂肪酸(FA)衍生的介体，包括内源性大麻酰胺(AEA)和2-花生酰甘油(2-AG)，它们是减肥药物开发的靶标，以及它们的同系物N-酰基乙醇胺(NAE)和2-单酰基甘油(2-MAG)，它们与食物摄取和能量代谢有关。体重和脂肪分布被认为是外周内源性大麻素水平的决定因素。我们的目的是调查不同人群中与循环NAE和2-MAG水平相关的体脂组成以外的因素。用LC-MS/MS测定了健康男性和女性(n = 195)的横断面样本在两天地中海饮食(n = 21)前后血浆NAE和2-MAG的含量。除N-油酰乙醇胺外，所有2-MAG和NAE的循环水平与体脂质量和内脏脂肪组织相关(0.26 <； r <； 0.54)。在脂肪质量升高的个体中，NAE水平升高，而在以内脏脂肪分布为主的个体中，2-MAG水平升高。饮食中特定FAs的摄入量与2-AG和omega-3-FA衍生的NAE或2-MAG相关，与身体脂肪分布无关。一些肠道细菌家族(例如韦龙科、消化链球菌科和阿克曼西亚科)与大多数NAE或由omega-3-FA衍生的2-MAG的变化有关，与脂肪质量和膳食FA摄入量无关。最后，为期两天的地中海饮食干预增加了循环中NAE和2-MAG的水平，这与FA摄入量的变化一致(p <； 0.01)。自我报告的摄入量和短期饮食干预增加了油酸、EPA和DHA的摄入量，以及某些肠道微生物区系与循环NAE和2-MAG无关的肥胖指标，从而突显了这些变量在确定人类内源性大麻素信号方面的潜在重要性。
The endocannabinoidome encompasses several fatty acid (FA)-derived mediators, including the endocannabinoid anandamide (AEA) and 2-arachidonoyl-glycerol (2-AG), which served as targets for anti-obesity drug development, and their congener N-acyl-ethanolamines (NAEs) and 2-monoacyl-glycerols (2‑MAGs), which are involved in food intake and energy metabolism. Body weight and fat distribution have been suggested as determinants of peripheral endocannabinoid levels. We aimed at investigating factors, beyond body fat composition, that are associated with circulating NAE and 2-MAG levels in a heterogeneous human population. Plasma NAEs and 2-MAGs were measured using LC–MS/MS in a cross-sectional sample of healthy men and women (n = 195) covering a wide range of BMI and individuals before and after a 2-day Mediterranean diet (n = 21). Circulating levels of all 2-MAGs and NAEs, other than N-oleoyl-ethanolamine (OEA), correlated with body fat mass and visceral adipose tissue (0.26 < r < 0.54). NAE levels were elevated in individuals with elevated fat mass, while 2-MAGs were increased in individuals with predominantly visceral body fat distribution. Dietary intakes of specific FAs were associated with 2-AG and omega-3-FA-derived NAEs or 2-MAGs, irrespective of the body fat distribution. Some gut bacterial families (e.g. Veillonellaceae, Peptostreptococcaceae and Akkermansiaceae) were associated with variations in most NAEs or omega-3-FA-derived 2‑MAGs, independently of fat mass and dietary FA intake. Finally, a 2-day Mediterranean diet intervention increased circulating levels of NAEs and 2-MAGs in agreement with changes in FA intake (p < 0.01). Self-reported intake and short-term dietary intervention increased in oleic acid and EPA and DHA intake as well as certain gut microbiota taxa are associated to circulating NAEs and 2‑MAGs independently of adiposity measures, thus highlighting the potential importance of these variables in determining endocannabinoidome signaling in humans.
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影响因子：
5.2
作者：
Cani PD;de Vos WM
通讯作者：
de Vos WM
影响因子：
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作者：
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64.8
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作者：
KEYS, A;MENOTTI, A;TOSHIMA, H
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