Zebrafish Larvae Position Tracker (Z-LaP Tracker): a high-throughput deep-learning behavioral approach for the identification of calcineurin pathway-modulating drugs using zebrafish larvae.
Zebrafish Larvae Position Tracker (Z-LaP Tracker): a high-throughput deep-learning behavioral approach for the identification of calcineurin pathway-modulating drugs using zebrafish larvae.
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DOI：
10.1038/s41598-023-30303-w
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发表时间：
2023-02-23
影响因子：
4.6
通讯作者：
Creton, Robbert
中科院分区：
文献类型：
--
作者：
Gore, Sayali V.;Kakodkar, Rohit;Hernandez, Thais Del Rosario;Edmister, Sara Tucker;Creton, Robbert
大脑功能研究在很大程度上依赖于行为的量化和分析。虽然行为可以有效地成像，但量化行为的特定方面是劳动密集型的，可能会引入个人偏见。深度学习和基于人工智能的工具的最新进展使人们能够精确跟踪不同环境中自由活动的动物的个体特征，而不需要任何标记。在目前的研究中，我们开发了斑马鱼幼体位置跟踪器(Z-LAP Tracker)，这是对无标记位置估计软件DeepLabCut的改进，用于在高通量384井环境中量化斑马鱼幼体的行为。我们利用我们的模式动物斑马鱼幼体的高对比度特征，包括眼睛和蛋黄来进行行为分析。使用这个实验装置，我们量化了相关的行为，其准确度与人类进行的分析类似。行为的改变被组织在行为档案中，并用K-均值和等级聚类分析进行检验。钙调神经磷酸酶抑制剂表现出明显的行为特征，表现为活动增加，声学超兴奋性，视觉引导行为减少，对声刺激的习惯性减少。开发的方法学被用来确定可能成为预防和治疗神经疾病的有前途的候选药物的“环孢素A”类药物。
Brain function studies greatly depend on quantification and analysis of behavior. While behavior can be imaged efficiently, the quantification of specific aspects of behavior is labor-intensive and may introduce individual biases. Recent advances in deep learning and artificial intelligence-based tools have made it possible to precisely track individual features of freely moving animals in diverse environments without any markers. In the current study, we developed Zebrafish Larvae Position Tracker (Z-LaP Tracker), a modification of the markerless position estimation software DeepLabCut, to quantify zebrafish larval behavior in a high-throughput 384-well setting. We utilized the high-contrast features of our model animal, zebrafish larvae, including the eyes and the yolk for our behavioral analysis. Using this experimental setup, we quantified relevant behaviors with similar accuracy to the analysis performed by humans. The changes in behavior were organized in behavioral profiles, which were examined by K-means and hierarchical cluster analysis. Calcineurin inhibitors exhibited a distinct behavioral profile characterized by increased activity, acoustic hyperexcitability, reduced visually guided behaviors, and reduced habituation to acoustic stimuli. The developed methodologies were used to identify ‘CsA-type’ drugs that might be promising candidates for the prevention and treatment of neurological disorders.
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影响因子：
4.6
作者：
Edmister, Sara Tucker;Hernandez, Thais Del Rosario;Ibrahim, Rahma;Brown, Cameron A.;Gore, Sayali, V;Kakodkar, Rohit;Kreiling, Jill A.;Creton, Robbert
通讯作者：
Creton, Robbert
DOI：
10.1523/jneurosci.1064-09.2009
发表时间：
2009-10-14
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Abdul HM;Sama MA;Furman JL;Mathis DM;Beckett TL;Weidner AM;Patel ES;Baig I;Murphy MP;LeVine H 3rd;Kraner SD;Norris CM
通讯作者：
Norris CM
DOI：
10.1002/alz.12325
发表时间：
2021-06
期刊：
Alzheimer's & dementia : the journal of the Alzheimer's Association
影响因子：
--
作者：
Datta D;Leslie SN;Wang M;Morozov YM;Yang S;Mentone S;Zeiss C;Duque A;Rakic P;Horvath TL;van Dyck CH;Nairn AC;Arnsten AFT
通讯作者：
Arnsten AFT
影响因子：
5.3
作者：
Green, Kim N.;Billings, Lauren M.;LaFerla, Frank M.
通讯作者：
LaFerla, Frank M.
钙稳态在阿尔茨海默氏病中的作用。
影响因子：
3.2
作者：
Ge M;Zhang J;Chen S;Huang Y;Chen W;He L;Zhang Y
通讯作者：
Zhang Y