Functional role for interleukin-1 in the injured peripheral taste system.
Functional role for interleukin-1 in the injured peripheral taste system.
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DOI：
10.1002/jnr.22798
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发表时间：
2012-04
影响因子：
4.2
通讯作者：
McCluskey, Lynnette Phillips
中科院分区：
文献类型：
--
作者：
Shi, Liqiao;He, Lianying;Sarvepalli, Padma;McCluskey, Lynnette Phillips
关键词：
外周味觉系统为研究神经损伤的后果提供了一个很好的模型，包括受损的神经和感觉细胞以及邻近的完整的神经细胞。对主要传入神经——鼓室索（CT）进行切片，可迅速向舌头两侧招募中性粒细胞。在完整的CT上，双侧中性粒细胞反应引起短暂的功能缺陷。随着巨噬细胞对损伤的反应，正常功能随后恢复。我们假设巨噬细胞产生促炎细胞因子白细胞介素(IL)-1，这有助于在附近损伤后维持正常的味觉功能。结果表明，损伤后第2天，舌侧损伤部位IL-1β蛋白水平显著升高。饮食中的钠缺乏可以抑制巨噬细胞的募集，从而抑制IL-1β的升高。IL-1β在多种细胞群中表达，包括味觉受体细胞和浸润性中性粒细胞和巨噬细胞。为了测试IL-1是否调节损伤后的味觉功能，我们用IL-1受体拮抗剂（IL-1 RA）阻断信号传导，并在完整的CT上记录味觉反应。这种治疗抑制了双侧巨噬细胞对损伤的反应，并削弱了完整CT的味觉反应。细胞因子在味觉系统中的作用在很大程度上尚未得到研究。这些结果表明，与IL-1在受损中枢神经系统（CNS）中的有害作用相反，IL-1对附近损伤后的味觉功能有有益的影响。
The peripheral taste system presents an excellent model for studying the consequences of neural injury, for the damaged nerve and sensory cells and the neighboring, intact neural cells. Sectioning a primary afferent nerve, the chorda tympani (CT), rapidly recruits neutrophils to both sides of the tongue. The bilateral neutrophil response induces transient functional deficits in the intact CT. Normal function is subsequently restored as macrophages respond to injury. We hypothesized that macrophages produce the proinflammatory cytokine, interleukin (IL)-1, which contributes to the maintenance of normal taste function after nearby injury. We demonstrate that IL-1β protein levels are significantly increased on the injured side of the tongue at day 2 after injury. Dietary sodium deficiency, a manipulation which prevents macrophage recruitment, inhibits the elevation in IL-1β. IL-1β was expressed in several cell populations, including taste receptor cells and infiltrating neutrophils and macrophages. To test whether IL-1 modulates taste function after injury, we blocked signaling with an IL-1 receptor antagonist (IL-1 RA) and recorded taste responses from the intact CT. This treatment inhibited the bilateral macrophage response to injury, and impaired taste responses in the intact CT. Cytokine actions in the taste system are largely unstudied. These results demonstrate that IL-1 has a beneficial effect on taste function after nearby injury, in contrast to its detrimental role in the injured central nervous system (CNS).
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影响因子：
4.2
作者：
Cavallin, MA;McCluskey, LP
通讯作者：
McCluskey, LP
外周味觉系统单侧神经损伤后趋化因子单核细胞趋化蛋白-1的上调
影响因子：
2.5
作者：
Cavallin, Melissa Ann;McCluskey, Lynnette Phillips
通讯作者：
McCluskey, Lynnette Phillips
影响因子：
5
作者：
Choi, Jae Young;Choi, Yoon-Seok;Yoon, Joo-Heon
通讯作者：
Yoon, Joo-Heon
DOI：
10.1523/jneurosci.3795-08.2008
发表时间：
2008-12-24
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Binshtok AM;Wang H;Zimmermann K;Amaya F;Vardeh D;Shi L;Brenner GJ;Ji RR;Bean BP;Woolf CJ;Samad TA
通讯作者：
Samad TA
DOI：
10.1152/ajpregu.00219.2009
发表时间：
2009-10-01
影响因子：
2.8
作者：
Guagliardo, Nick A.;West, Katie Nicole;Hill, David L.
通讯作者：
Hill, David L.