Anti-inflammatory Lactobacillus rhamnosus CNCM I-3690 strain protects against oxidative stress and increases lifespan in Caenorhabditis elegans.
Anti-inflammatory Lactobacillus rhamnosus CNCM I-3690 strain protects against oxidative stress and increases lifespan in Caenorhabditis elegans.
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DOI：
10.1371/journal.pone.0052493
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Ramón D
中科院分区：
文献类型：
--
作者：
Grompone G;Martorell P;Llopis S;González N;Genovés S;Mulet AP;Fernández-Calero T;Tiscornia I;Bollati-Fogolín M;Chambaud I;Foligné B;Montserrat A;Ramón D
许多研究表明，抗氧化应激对保持健康和减少衰老的不利影响至关重要。因此，使用抗氧化剂食品级化合物或食品的营养干预目前是帮助改善老年人健康和生活质量的一个有趣的选择。在食物中施用的活乳酸菌（LAB），如益生菌，可能是良好的抗氧化剂候选者。然而，关于LAB诱导的氧化应激保护的信息是稀缺的。为了鉴定和表征新的潜在抗氧化益生菌菌株，我们开发了一种新的功能筛选方法，使用线虫秀丽隐杆线虫作为宿主。C.用不同的LAB菌株（总共78种）喂养线虫，并在氧化应激（3 mM和5 mMH 2 O2）后评估线虫的生存力。一种被鉴定为鼠李糖乳杆菌CNCM I-3690的菌株通过将其生存能力提高30%来保护蠕虫，并且还将蠕虫的平均寿命提高20%。此外，还对C.用这种菌株喂养的秀丽线虫表明，寿命的增加与β-16/胰岛素样途径的差异表达相关，该途径在人类中高度保守。当与HT-29细胞共培养、受促炎细胞因子刺激以及在LPS存在下与HT-29细胞和DC共培养系统时，该菌株也具有明显的抗炎特性。最后，这种乳酸杆菌菌株减少了结肠炎小鼠模型中的炎症。这一工作表明C. elegans是一种快速、预测性和方便的筛选工具，用于识别新的潜在抗氧化益生菌菌株，以供随后用于人类。
Numerous studies have shown that resistance to oxidative stress is crucial to stay healthy and to reduce the adverse effects of aging. Accordingly, nutritional interventions using antioxidant food-grade compounds or food products are currently an interesting option to help improve health and quality of life in the elderly. Live lactic acid bacteria (LAB) administered in food, such as probiotics, may be good antioxidant candidates. Nevertheless, information about LAB-induced oxidative stress protection is scarce. To identify and characterize new potential antioxidant probiotic strains, we have developed a new functional screening method using the nematode Caenorhabditis elegans as host. C. elegans were fed on different LAB strains (78 in total) and nematode viability was assessed after oxidative stress (3 mM and 5 mM H2O2). One strain, identified as Lactobacillus rhamnosus CNCM I-3690, protected worms by increasing their viability by 30% and, also, increased average worm lifespan by 20%. Moreover, transcriptomic analysis of C. elegans fed with this strain showed that increased lifespan is correlated with differential expression of the DAF-16/insulin-like pathway, which is highly conserved in humans. This strain also had a clear anti-inflammatory profile when co-cultured with HT-29 cells, stimulated by pro-inflammatory cytokines, and co-culture systems with HT-29 cells and DC in the presence of LPS. Finally, this Lactobacillus strain reduced inflammation in a murine model of colitis. This work suggests that C. elegans is a fast, predictive and convenient screening tool to identify new potential antioxidant probiotic strains for subsequent use in humans.
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影响因子：
12.3
作者：
Gentleman RC;Carey VJ;Bates DM;Bolstad B;Dettling M;Dudoit S;Ellis B;Gautier L;Ge Y;Gentry J;Hornik K;Hothorn T;Huber W;Iacus S;Irizarry R;Leisch F;Li C;Maechler M;Rossini AJ;Sawitzki G;Smith C;Smyth G;Tierney L;Yang JY;Zhang J
通讯作者：
Zhang J
影响因子：
6.1
作者：
Frankel, Edwin N.
通讯作者：
Frankel, Edwin N.
DOI：
10.1111/j.1532-5415.1972.tb00787.x
发表时间：
1972-01-01
影响因子：
6.3
作者：
HARMAN, D
通讯作者：
HARMAN, D
影响因子：
4.9
作者：
Han, Wei;Mercenier, Annick;Fioramonti, Jean
通讯作者：
Fioramonti, Jean
DOI：
10.1083/jcb.201102095
发表时间：
2011-07-11
期刊：
The Journal of cell biology
影响因子：
--
作者：
Finkel T
通讯作者：
Finkel T