Heterogeneous feeding patterns of the dengue vector, Aedes aegypti, on individual human hosts in rural Thailand.
Heterogeneous feeding patterns of the dengue vector, Aedes aegypti, on individual human hosts in rural Thailand.
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DOI：
10.1371/journal.pntd.0003048
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发表时间：
2014-08
影响因子：
3.8
通讯作者：
Scott TW
中科院分区：
文献类型：
--
作者：
Harrington LC;Fleisher A;Ruiz-Moreno D;Vermeylen F;Wa CV;Poulson RL;Edman JD;Clark JM;Jones JW;Kitthawee S;Scott TW
蚊子叮咬的频率和叮咬在不同人群中的分布情况可能会产生重大的流行病学影响。更好地了解蚊子病媒-人的相互作用将完善支持病原体传播的昆虫学过程的知识，并可以揭示最大限度地减少风险和打破病原体传播周期的目标。我们使用人DNA血餐分析登革热病毒（DENV）载体，埃及伊蚊，量化其与人类宿主的接触，并推断其吸血行为的流行病学意义。我们通过宿主年龄、大小、蚊子年龄和每个人被叮咬的次数来确定不同人被叮咬的数量、叮咬频率。在收集的3,677只饱食的蚊子和1,186份完整的DNA图谱中，只有420份食物与研究地区的人相匹配，这表明Ae。埃及人靠在社区间流动的人为生，从事日常业务。10-13%的饱腹蚊子会吸食一个以上的人。在登革热高传播季节和低传播季节之间没有检测到叮咬率差异。我们估计，43-46%的饱血蚊子在每个生殖营养周期内叮咬一个以上的人。大多数多餐来自蚊子收集屋的居民或邻居。年龄≤25岁的人被咬伤的次数少于老年人。有些宿主被频繁取食，有三个宿主被咬了九次。蚊子和人类的相互作用网络揭示了具有生物学意义的吸血热点，包括社区市场。高多喂养率和喂养社区游客可能是DENV有效传播和快速传播的重要特征。这些结果有助于解释为什么单单减少病媒种群很难预防登革热，并支持对蚊子喂养行为进行更多研究的论点，当与对人类行为的更深入了解相结合时，将改进对登革热控制风险和策略的估计。登革热是一种潜在的致命感染，每年影响数亿人的生命，是由蚊子吸血时传播的病毒引起的。由于没有疫苗或治疗方法可用于商业，了解与病毒暴露相关的潜在因素对于开发更有效的登革热干预措施至关重要。我们在泰国的一个流行地区进行了一项研究，那里的传播率很高，儿童预计将成为宿主人口中的非免疫、扩增部分。我们检查了Ae。通过匹配吸血蚊子中的人类DNA图谱来研究该地区的居民。少量的食物与研究地区的人相匹配，这表明蚊子以在社区中短暂移动的人为食。25岁以下的人被咬伤的频率低于老年人。我们构建了网络模型，以探讨蚊子喂养的“热点”的存在，并检测到一个研究村在登革热高传播季节的当地市场的“热点”。我们的研究结果提供了关于登革热病媒喂养模式的新细节，并强调需要对病媒喂养和人类行为以及病毒传播模式进行综合研究，以便更好地了解登革热的传播效率和传播。
Mosquito biting frequency and how bites are distributed among different people can have significant epidemiologic effects. An improved understanding of mosquito vector-human interactions would refine knowledge of the entomological processes supporting pathogen transmission and could reveal targets for minimizing risk and breaking pathogen transmission cycles. We used human DNA blood meal profiling of the dengue virus (DENV) vector, Aedes aegypti, to quantify its contact with human hosts and to infer epidemiologic implications of its blood feeding behavior. We determined the number of different people bitten, biting frequency by host age, size, mosquito age, and the number of times each person was bitten. Of 3,677 engorged mosquitoes collected and 1,186 complete DNA profiles, only 420 meals matched people from the study area, indicating that Ae. aegypti feed on people moving transiently through communities to conduct daily business. 10–13% of engorged mosquitoes fed on more than one person. No biting rate differences were detected between high- and low-dengue transmission seasons. We estimate that 43–46% of engorged mosquitoes bit more than one person within each gonotrophic cycle. Most multiple meals were from residents of the mosquito collection house or neighbors. People ≤25 years old were bitten less often than older people. Some hosts were fed on frequently, with three hosts bitten nine times. Interaction networks for mosquitoes and humans revealed biologically significant blood feeding hotspots, including community marketplaces. High multiple-feeding rates and feeding on community visitors are likely important features in the efficient transmission and rapid spread of DENV. These results help explain why reducing vector populations alone is difficult for dengue prevention and support the argument for additional studies of mosquito feeding behavior, which when integrated with a greater understanding of human behavior will refine estimates of risk and strategies for dengue control. Dengue, a potentially lethal infection impacting hundreds of millions of human lives annually, is caused by viruses transmitted during mosquito blood feeding. With no vaccine or treatment commercially available, understanding the underlying factors linked to virus exposure is critical for developing more effective dengue interventions. We conducted a study in an endemic region of Thailand where transmission is high and children are expected to be the non-immune, amplifying portion of the host population. We examined Ae. aegypti feeding patterns and risk by matching human DNA profiles in blood-fed mosquitoes to study area residents. A small number of meals matched people from the study area, suggesting that mosquitoes feed on people moving transiently through communities. People under 25 years of age were bitten less frequently than older people. We constructed network models to explore the presence of mosquito feeding “hotspots” and detected a local market “hotspot” in one study village during the high dengue transmission season. Our results provide new details on dengue vector feeding patterns and highlight the need to conduct integrated studies of vector feeding and human behavior, and virus transmission patterns in order to better understand the dengue transmission efficiency and spread.
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影响因子：
9.8
作者：
通讯作者：
--
影响因子：
5
作者：
Endy, TP;Chunsuttiwat, S;Ennis, FA
通讯作者：
Ennis, FA
DOI：
10.4269/ajtmh.1969.18.997
发表时间：
1969-01-01
影响因子：
3.3
作者：
HALSTEAD, SB;SCANLON, JE;UDOMSAKDI, S
通讯作者：
UDOMSAKDI, S
影响因子：
3.3
作者：
Chung, YK;Pang, FY
通讯作者：
Pang, FY
影响因子：
64.8
作者：
Lloyd-Smith JO;Schreiber SJ;Kopp PE;Getz WM
通讯作者：
Getz WM