Eight-Channel AC Magnetosusceptometer of Magnetic Nanoparticles for High-Throughput and Ultra-High-Sensitivity Immunoassay.
Eight-Channel AC Magnetosusceptometer of Magnetic Nanoparticles for High-Throughput and Ultra-High-Sensitivity Immunoassay.
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DOI：
10.3390/s18041043
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发表时间：
2018-03-30
期刊：
影响因子：
--
通讯作者：
Yang SY
中科院分区：
文献类型：
--
作者：
Chieh JJ;Wei WC;Liao SH;Chen HH;Lee YF;Lin FC;Chiang MH;Chiu MJ;Horng HE;Yang SY
研制了一种用于免疫磁性还原（IMR）的抗体功能化磁性纳米粒子（MNP）交流磁共振仪。磁敏仪采用了高灵敏度、高临界温度的超导量子干涉器件。在血清中可检测到早期神经退行性疾病的生物标志物的微小水平，但测量每个生物标志物需要大约4小时。因此，在本研究中开发了一个八通道平台，以满足阿尔茨海默病的最低筛查要求。每份人体标本的三种生物标志物（即Aβ40、Aβ42和tau蛋白）测量到两个一致的结果。本文介绍了仪器配置以及关键特征，例如通道之间的低噪声水平变化、高信噪比以及生物标志物IMR值的变异系数。本研究还证明了该仪器对三种生物标志物的灵敏度水平以及与以前的单通道和四通道平台相比显著缩短的总测量时间。因此，八通道仪器可以作为一个强有力的工具，临床高通量筛选阿尔茨海默病。
An alternating-current magnetosusceptometer of antibody-functionalized magnetic nanoparticles (MNPs) was developed for immunomagnetic reduction (IMR). A high-sensitivity, high-critical-temperature superconducting quantum interference device was used in the magnetosusceptometer. Minute levels of biomarkers of early-stage neurodegeneration diseases were detectable in serum, but measuring each biomarker required approximately 4 h. Hence, an eight-channel platform was developed in this study to fit minimal screening requirements for Alzheimer’s disease. Two consistent results were measured for three biomarkers, namely Aβ40, Aβ42, and tau protein, per human specimen. This paper presents the instrument configuration as well as critical characteristics, such as the low noise level variations among channels, a high signal-to-noise ratio, and the coefficient of variation for the biomarkers’ IMR values. The instrument’s ultrahigh sensitivity levels for the three biomarkers and the substantially shorter total measurement time in comparison with the previous single- and four-channels platforms were also demonstrated in this study. Thus, the eight-channel instrument may serve as a powerful tool for clinical high-throughput screening of Alzheimer’s disease.
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影响因子：
5
作者：
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通讯作者：
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Semmler, W
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通讯作者：
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