Identification of small RNAs associated with RNA chaperone Hfq reveals a new stress response regulator in Actinobacillus pleuropneumoniae.
Identification of small RNAs associated with RNA chaperone Hfq reveals a new stress response regulator in Actinobacillus pleuropneumoniae.
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DOI：
10.3389/fmicb.2022.1017278
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发表时间：
2022
影响因子：
5.2
通讯作者：
Bazzolli, Denise Mara Soares
中科院分区：
文献类型：
--
作者：
da Silva, Giarla Cunha;Rossi, Ciro Cesar;Rosa, Jessica Nogueira;Sanches, Newton Moreno;Cardoso, Daniela Lopes;Li, Yanwen;Witney, Adam A. A.;Gould, Kate A. A.;Fontes, Patricia Pereira;Callaghan, Anastasia J. J.;Bosse, Janine Therese;Langford, Paul Richard;Bazzolli, Denise Mara Soares
关键词：
RNA分子伴侣Hfq促进小RNA（sRNA）与同源mRNA的结合，控制细菌表型的表达。胸膜肺炎放线杆菌hfq突变株在猪中的毒力减弱，形成生物膜的能力受损，对应激更敏感，但对sRNA参与程度的了解有限。这里，使用A.在胸膜肺炎菌株MIDG 2331（血清型8）中，通过与Hfq的免疫共沉淀鉴定了14种sRNA，并且通过北方印迹证实了鉴定为反式作用sRNA的8种sRNA的表达。我们专注于这些sRNA之一，命名为Rna 01，含有一个假定的启动子RpoE（应激调节子）识别。rna 01基因敲除突变体和rna 01与hfq基因双敲除突变体均具有降低的生物膜形成和溶血活性，大蜡螟的毒力减弱，改变的应激敏感性，以及改变的外膜蛋白谱。rna 01影响G.梅隆内拉rna 01和推定的同源mRNA靶标的qRT-PCR分析表明，Rna 01与胞质外应激反应相关。这项工作增加了我们对Hfq依赖的sRNAs对A.胸膜肺炎
The RNA chaperone Hfq promotes the association of small RNAs (sRNAs) with cognate mRNAs, controlling the expression of bacterial phenotype. Actinobacillus pleuropneumoniae hfq mutants strains are attenuated for virulence in pigs, impaired in the ability to form biofilms, and more susceptible to stress, but knowledge of the extent of sRNA involvement is limited. Here, using A. pleuropneumoniae strain MIDG2331 (serovar 8), 14 sRNAs were identified by co-immunoprecipitation with Hfq and the expression of eight, identified as trans-acting sRNAs, were confirmed by Northern blotting. We focused on one of these sRNAs, named Rna01, containing a putative promoter for RpoE (stress regulon) recognition. Knockout mutants of rna01 and a double knockout mutant of rna01 and hfq, both had decreased biofilm formation and hemolytic activity, attenuation for virulence in Galleria mellonella, altered stress susceptibility, and an altered outer membrane protein profile. Rna01 affected extracellular vesicle production, size and toxicity in G. mellonella. qRT-PCR analysis of rna01 and putative cognate mRNA targets indicated that Rna01 is associated with the extracytoplasmic stress response. This work increases our understanding of the multilayered and complex nature of the influence of Hfq-dependent sRNAs on the physiology and virulence of A. pleuropneumoniae.
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影响因子：
12.3
作者：
Buels R;Yao E;Diesh CM;Hayes RD;Munoz-Torres M;Helt G;Goodstein DM;Elsik CG;Lewis SE;Stein L;Holmes IH
通讯作者：
Holmes IH
RNA伴侣Hfq与第二信使c-di-GMP之间的多重调控关系
影响因子：
5.2
作者：
Fu Y;Yu Z;Zhu L;Li Z;Yin W;Shang X;Chou SH;Tan Q;He J
通讯作者：
He J
影响因子：
3.7
作者：
Basavanna S;Chimalapati S;Maqbool A;Rubbo B;Yuste J;Wilson RJ;Hosie A;Ogunniyi AD;Paton JC;Thomas G;Brown JS
通讯作者：
Brown JS
影响因子：
4.8
作者：
Böhm, A;Diez, J;Boos, W
通讯作者：
Boos, W
DOI：
10.1128/genomea.01667-15
发表时间：
2016-01-28
期刊：
Genome announcements
影响因子：
--
作者：
Bossé JT;Chaudhuri RR;Li Y;Leanse LG;Fernandez Crespo R;Coupland P;Holden MT;Bazzolli DM;Maskell DJ;Tucker AW;Wren BW;Rycroft AN;Langford PR
通讯作者：
Langford PR