Classification of marine diatoms using pigment ratio suites
Classification of marine diatoms using pigment ratio suites
复制标题
使用色素比例套件对海洋硅藻进行分类
DOI：
10.1007/s00343-011-0202-8
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发表时间：
2011-08
影响因子：
--
通讯作者：
Yao Peng
中科院分区：
文献类型：
--
作者：
Liu Shuxia;Yu Zhigang;Zhen Yu;Deng Chunmei;Yao Peng
硅藻广泛分布在许多温带地区，一些物种经常在春季大规模开花。因此，监测特定硅藻属或种的变化对于研究海洋生态系统中浮游植物种群动态是必要的。为了测试色素比例是否可用于鉴定低于分类水平的硅藻，我们使用高效液相色谱 (HPLC) 分析了从中国海域分离的 14 种/品系的硅藻。我们将所有色素浓度标准化为总叶绿素，计算色素与叶绿素的比率，并计算辅助色素（或色素总和）之间的比率。聚类分析表明，根据三种辅助色素的比例，这些硅藻可分为四类：叶绿素2：叶绿素1、岩藻黄质：总叶绿素和二氮黄质：二毒素。分类结果与生物分类学的结果吻合良好。为了测试分类的稳定性，计算了在不同光强度下培养的一个物种和五个新物种/菌株的色素数据并用于判别分析。结果表明，对于不同光强下培养的物种色素比率的变化，使用色素比率套件对硅藻物种进行分类是稳定的。然而，新物种的引入可能会混淆当前计划中的分类。使用色素比例套件对海洋硅藻进行分类对于类以下分类水平的浮游植物精细化学分类学具有潜在价值，并将推进浮游植物种群动态和海洋生态学的研究。
Diatoms are widely distributed in many temperate areas and some species frequently form extensive blooms in spring. Hence, monitoring the variations of specific genera or species of diatoms is necessary for studying phytoplankton population dynamics in marine ecosystems. To test whether pigment ratios can be used to identify diatoms at a below-class taxonomic level, we analyzed 14 species/strains of diatoms isolated from Chinese seas using high performance liquid chromatography (HPLC). We normalized all pigment concentrations to total chlorophyllato calculate the ratios of pigment to chlorophylla, and calculated the ratios between accessory pigments (or pigment sums). Cluster analysis indicated that these diatoms could be classified into four clusters in terms of three accessory pigment ratios: chlorophyllc2: chlorophyllc1, fucoxanthin:total chlorophyllcand diadinoxanthin:diatoxanthin. The classification results matched well with those of biological taxonomy. To test the stability of the classification, pigment data from one species, cultured under different light intensities, and five new species/strains were calculated and used for discriminant analysis. The results show that the classification of diatom species using pigment ratio suites was stable for the variations of pigment ratios of species cultured in different light intensities. The introduction of new species, however, may confuse the classification within the current scheme. Classification of marine diatoms using pigment ratio suites is potentially valuable for the fine chemotaxonomy of phytoplankton at taxonomic levels below class and would advance studies on phytoplankton population dynamics and marine ecology.
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DOI：
10.1016/s0967-0645(01)00119-9
发表时间：
2002
影响因子：
3
作者：
Jaime Rodríguez;F. Jiménez-Gómez;J. M. Blanco;F. Figueroa
通讯作者：
Jaime Rodríguez;F. Jiménez-Gómez;J. M. Blanco;F. Figueroa
DOI：
10.1017/s002531540001211x
发表时间：
1946-07
影响因子：
1.2
作者：
D. P. Wilson
通讯作者：
D. P. Wilson
影响因子：
2.9
作者：
R. Airs;C. Llewellyn
通讯作者：
R. Airs;C. Llewellyn
影响因子：
2.5
作者：
Zapata, M;Rodríguez, F;Garrido, JL
通讯作者：
Garrido, JL
影响因子：
2.9
作者：
E. Örnólfsdóttir;J. Pinckney;P. Tester
通讯作者：
E. Örnólfsdóttir;J. Pinckney;P. Tester