Bacterial urease and its role in long-lasting human diseases.
Bacterial urease and its role in long-lasting human diseases.
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DOI：
10.2174/138920312804871094
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发表时间：
2012-12
影响因子：
2.8
通讯作者：
Kaca W
中科院分区：
文献类型：
--
作者：
Konieczna I;Zarnowiec P;Kwinkowski M;Kolesinska B;Fraczyk J;Kaminski Z;Kaca W
尿素酶是一种存在于多种病原菌中的毒力因子。它在宿主生物体的定殖和组织中细菌细胞的维持中是必需的。由于其酶活性，脲酶对人体细胞具有毒性作用。尿素溶解活性的存在是许多细菌感染的重要标志。尿素酶也是一种免疫原性蛋白质，可被人血清中的抗体识别。这种抗体的存在与几种长期疾病的进展有关，如类风湿性关节炎，动脉粥样硬化或尿路感染。在细菌尿素酶中，可能存在具有与人类蛋白质相似的序列和/或结构的基序。这种现象被称为分子拟态，导致自身抗体的出现，参与宿主分子的破坏。细菌蛋白中抗体结合基序（表位）的检测是一个复杂的过程。然而，合成肽库等有机化学工具对于表位作图和血清学研究都很有帮助。 在这篇综述中，我们提出了一个合成报告的细菌尿素酶的分子组织-遗传以及结构。我们表征用于检测尿素酶和尿素分解活性的方法，包括应用于疾病诊断过程和尿素酶表位化学合成的技术。本文还对细菌性尿素酶对人体的毒性作用以及抗尿素酶抗体在长期疾病中的发生进行了综述。
Urease is a virulence factor found in various pathogenic bacteria. It is essential in colonization of a host organism and in maintenance of bacterial cells in tissues. Due to its enzymatic activity, urease has a toxic effect on human cells. The presence of ureolytic activity is an important marker of a number of bacterial infections. Urease is also an immunogenic protein and is recognized by antibodies present in human sera. The presence of such antibodies is connected with progress of several long-lasting diseases, like rheumatoid arthritis, atherosclerosis or urinary tract infections. In bacterial ureases, motives with a sequence and/or structure similar to human proteins may occur. This phenomenon, known as molecular mimicry, leads to the appearance of autoantibodies, which take part in host molecules destruction. Detection of antibodies-binding motives (epitopes) in bacterial proteins is a complex process. However, organic chemistry tools, such as synthetic peptide libraries, are helpful in both, epitope mapping as well as in serologic investigations. In this review, we present a synthetic report on a molecular organization of bacterial ureases - genetic as well as structural. We characterize methods used in detecting urease and ureolytic activity, including techniques applied in disease diagnostic processes and in chemical synthesis of urease epitopes. The review also provides a summary of knowledge about a toxic effect of bacterial ureases on human body and about occurrence of anti-urease antibodies in long-lasting diseases.
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影响因子：
2.7
作者：
Ay, Bernhard;Landgraf, Katja;Boisguerin, Prisca
通讯作者：
Boisguerin, Prisca
影响因子：
3.1
作者：
Dupuy, B;Daube, G;Cole, ST
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影响因子：
4.8
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Espanel, X;Wälchli, S;van Huijsduijnen, RH
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van Huijsduijnen, RH
影响因子：
3.2
作者：
CLEMENS, DL;LEE, BY;HORWITZ, MA
通讯作者：
HORWITZ, MA
影响因子：
2.2
作者：
Burnie, JP;AlDughaym, A
通讯作者：
AlDughaym, A