The Escherichia coli TatABC system and a Bacillus subtilis TatAC-type system recognise three distinct targeting determinants in twin-arginine signal peptides.
The Escherichia coli TatABC system and a Bacillus subtilis TatAC-type system recognise three distinct targeting determinants in twin-arginine signal peptides.
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大肠杆菌 TatABC 系统和枯草芽孢杆菌 TatAC 型系统可识别双精氨酸信号肽中的三个不同的靶向决定簇。
DOI：
10.1016/j.jmb.2007.09.087
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发表时间：
2008
影响因子：
5.6
通讯作者：
Mendel S
中科院分区：
文献类型：
--
作者：
Mendel S
The Tat system transports folded proteins across bacterial and thylakoid membranes. In Gram-negative organisms, it is encoded by tatABC genes and the system recognizes substrates bearing signal peptides with a conserved twin-arginine motif. Most Gram-positive organisms lack a tatB gene, indicating major differences in organisation and/or mechanism. Here, we have characterized the essential targeting determinants that are recognized by a Bacillus subtilis TatAC-type system, TatAdCd. Substitution by lysine of either of the twin-arginine residues in the TorA signal peptide can be tolerated, but the presence of twin-lysine residues blocks export completely. We show that additional determinants can be as important as the twin-arginine motif. Replacement of the −1 serine by alanine, in either the TorA or DmsA signal peptide, almost blocks export by either the B. subtilis TatAdCd or Escherichia coli TatABC systems, firmly establishing the importance of this −1 residue in these signal peptides. Surprisingly, the +2 leucine in the DmsA signal peptide (sequence SRRGLV) appears to play an equally important role and substitution by alanine or phenylalanine blocks export by both the B. subtilis and E. coli systems. These data identify three distinct determinants, whose importance varies depending on the signal peptide in question. The data also show that the B. subtilis TatAdCd and E. coli TatABC systems recognize very similar determinants within their target peptides, and exhibit surprisingly similar responses to mutations within these determinants.
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影响因子：
56.9
作者：
Settles, AM;Yonetani, A;Martienssen, R
通讯作者：
Martienssen, R
影响因子：
3.5
作者：
Ray, N;Oates, J;Robinson, C
通讯作者：
Robinson, C
DOI：
10.1073/pnas.76.9.4530
发表时间：
1979-01-01
影响因子：
11.1
作者：
CASADABAN, MJ;COHEN, SN
通讯作者：
COHEN, SN
影响因子：
3.5
作者：
Brink, S;Bogsch, EG;Robinson, C
通讯作者：
Robinson, C
DOI：
10.1093/protein/10.8.975
发表时间：
1997-08-01
期刊：
PROTEIN ENGINEERING
影响因子：
--
作者：
Voss, S;Skerra, A
通讯作者：
Skerra, A