Intranasal immunization with plasmid DNA encoding spike protein of SARS-coronavirus/polyethylenimine nanoparticles elicits antigen-specific humoral and cellular immune responses.
Intranasal immunization with plasmid DNA encoding spike protein of SARS-coronavirus/polyethylenimine nanoparticles elicits antigen-specific humoral and cellular immune responses.
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DOI：
10.1186/1471-2172-11-65
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发表时间：
2010-12-31
期刊：
影响因子：
3
通讯作者：
Yun CH
中科院分区：
文献类型：
--
作者：
Shim BS;Park SM;Quan JS;Jere D;Chu H;Song MK;Kim DW;Jang YS;Yang MS;Han SH;Park YH;Cho CS;Yun CH
用严重急性呼吸综合征（SARS）冠状病毒（CoV）的刺突蛋白（S）免疫小鼠，已知可产生中和抗体并预防SARS-CoV引起的感染。聚乙烯亚胺25K（PEI）是一种阳离子聚合物，可有效递送质粒DNA。在本研究中，通过鼻内（i.n.）研究了PEI/pci-S复合物形式的SARS DNA疫苗（pci-S）的免疫途径。PEI/pci-S纳米颗粒的大小约为194.7 ± 99.3 nm，并在体外证实了S mRNA和蛋白的表达。用i.n. PEI/pci-S纳米粒在血清中产生的S特异性IgG1和在肺洗液中产生的粘膜分泌型IgA显著高于单独用pci-S处理的小鼠（P <0.05）。与单独用pci-S攻击的小鼠相比，在PEI/pci-S接种的小鼠中发现的B220+细胞的数量增加。PEI/pci-S疫苗接种后，小鼠颈淋巴结中CD11c+树突状细胞表面共刺激分子（CD80和CD86）和II类主要组织相容性复合物分子（I-Ad）表达增加。PEI/pci-S免疫小鼠产生IFN-γ、TNF-α和IL-2的细胞百分比高于对照小鼠。这些结果表明，用PEI/pci-S纳米颗粒鼻内免疫诱导抗原特异性体液和细胞免疫应答。
Immunization with the spike protein (S) of severe acute respiratory syndrome (SARS)-coronavirus (CoV) in mice is known to produce neutralizing antibodies and to prevent the infection caused by SARS-CoV. Polyethylenimine 25K (PEI) is a cationic polymer which effectively delivers the plasmid DNA. In the present study, the immune responses of BALB/c mice immunized via intranasal (i.n.) route with SARS DNA vaccine (pci-S) in a PEI/pci-S complex form have been examined. The size of the PEI/pci-S nanoparticles appeared to be around 194.7 ± 99.3 nm, and the expression of the S mRNA and protein was confirmed in vitro. The mice immunized with i.n. PEI/pci-S nanoparticles produced significantly (P < 0.05) higher S-specific IgG1 in the sera and mucosal secretory IgA in the lung wash than those in mice treated with pci-S alone. Compared to those in mice challenged with pci-S alone, the number of B220+ cells found in PEI/pci-S vaccinated mice was elevated. Co-stimulatory molecules (CD80 and CD86) and class II major histocompatibility complex molecules (I-Ad) were increased on CD11c+ dendritic cells in cervical lymph node from the mice after PEI/pci-S vaccination. The percentage of IFN-γ-, TNF-α- and IL-2-producing cells were higher in PEI/pci-S vaccinated mice than in control mice. These results showed that intranasal immunization with PEI/pci-S nanoparticles induce antigen specific humoral and cellular immune responses.
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影响因子：
64.8
作者：
Li W;Moore MJ;Vasilieva N;Sui J;Wong SK;Berne MA;Somasundaran M;Sullivan JL;Luzuriaga K;Greenough TC;Choe H;Farzan M
通讯作者：
Farzan M
通过用N，M和E基因诱导DNA疫苗对小鼠SARS-COV的DNA疫苗接种。
DOI：
10.1016/j.bbrc.2005.01.048
发表时间：
2005-03-25
影响因子：
3.1
作者：
Jin H;Xiao C;Chen Z;Kang Y;Ma Y;Zhu K;Xie Q;Tu Y;Yu Y;Wang B
通讯作者：
Wang B
影响因子：
5.4
作者：
Tumpey, TM;Renshaw, M;Katz, JM
通讯作者：
Katz, JM
DOI：
10.1073/pnas.90.9.4156
发表时间：
1993-05-01
影响因子：
11.1
作者：
WANG, B;UGEN, KE;WEINER, DB
通讯作者：
WEINER, DB
影响因子：
30.5
作者：
Wu, CY;Kirman, JR;Seder, RA
通讯作者：
Seder, RA