Widening spinal injury research to consider all supraspinal cell types: Why we must and how we can.
Widening spinal injury research to consider all supraspinal cell types: Why we must and how we can.
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DOI：
10.1016/j.expneurol.2021.113862
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发表时间：
2021-12
影响因子：
5.3
通讯作者：
Tsoulfas P
中科院分区：
文献类型：
--
作者：
Blackmore M;Batsel E;Tsoulfas P
棘上神经节由数十个神经元群体组成，这些神经元群体将轴突从大脑投射到脊髓，以影响广泛的运动、自主和感觉功能。脊髓上神经元的复杂性和广泛分布带来了巨大的技术挑战，导致大多数脊髓损伤研究集中在少数几个主要通路上，如皮质脊髓、红核脊髓和神经脊椎。对于许多携带信息以调节或补偿这些主要通路的额外人群，或者携带对脊柱损伤患者具有高价值的自主神经前状态和其他信息的人群，我们了解的要少得多。然而，随着技术发展的融合，现在可以对脊髓损伤进行完整的连接体研究。改进的病毒标记、组织清理和自动注册到3D图谱可以量化整个小鼠大脑的脊髓上神经元，为以前所未有的规模跟踪对损伤和治疗的反应提供了一种实用的手段。在这里，我们讨论了在脊髓损伤研究中扩展连接组分析的必要性，通过讨论一个新的基于网络的研究脊髓上神经元的新的网络资源来说明这种潜力，并强调了连接组分析的未来前景。
The supraspinal connectome consists of dozens of neuronal populations that project axons from the brain to the spinal cord to influence a wide range of motor, autonomic, and sensory functions. The complexity and wide distribution of supraspinal neurons present significant technical challenges, leading most spinal cord injury research to focus on a handful of major pathways such as the corticospinal, rubrospinal, and raphespinal. Much less is known about many additional populations that carry information to modulate or compensate for these main pathways, or which carry pre-autonomic and other information of high value to individuals with spinal injury. A confluence of technical developments, however, now enables a whole-connectome study of spinal cord injury. Improved viral labeling, tissue clearing, and automated registration to 3D atlases can quantify supraspinal neurons throughout the murine brain, offering a practical means to track responses to injury and treatment on an unprecedented scale. Here we discuss the need for expanded connectome-wide analyses in spinal injury research, illustrate the potential by discussing a new web-based resource for brain-wide study of supraspinal neurons, and highlight future prospects for connectome analyses.
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影响因子：
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