Advances in proteomic phenotyping of microglia in neurodegeneration.
Advances in proteomic phenotyping of microglia in neurodegeneration.
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小胶质细胞是中枢神经系统（CNS）中动态的常驻免疫细胞，能够感知、监测其环境的变化并作出反应。在疾病状态下，小胶质细胞从稳态转变为多种分子表型状态，在神经系统疾病的发病机制中发挥着复杂的因果作用，小胶质细胞基因被确定为神经退行性疾病的遗传风险因素就证明了这一点。虽然对人类和动物模型中枢神经系统中小胶质细胞的转录组分析取得了进展，提供了变革性的见解，但转录组对蛋白质组的反映程度有限。因此，小胶质细胞的蛋白质组分析更有可能提供具有功能和治疗相关性的靶点。在这篇综述中，我们讨论了在阿尔茨海默病这一典型神经退行性疾病背景下，从小胶质细胞转录组研究中获得的分子见解，并强调了对来自体内模型系统和人脑的小胶质细胞进行蛋白质组分析的现有和新兴方法。
Microglia are dynamic resident immune cells of the central nervous system (CNS) that sense, survey, and respond to changes in their environment. In disease states, microglia transform from homeostatic to diverse molecular phenotypic states that play complex and causal roles in neurologic disease pathogenesis, as evidenced by the identification of microglial genes as genetic risk factors for neurodegenerative disease. While advances in transcriptomic profiling of microglia from the central nervous system of humans and animal models have provided transformative insights, the transcriptome is only modestly reflective of the proteome. Proteomic profiling of microglia is therefore more likely to provide functionally and therapeutically relevant targets. In this review, we discuss molecular insights gained from transcriptomic studies of microglia in the context of Alzheimer’s disease as a prototypic neurodegenerative disease, and highlight existing and emerging approaches for proteomic profiling of microglia derived from in vivo model systems and human brain.
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DOI：
10.1038/nrneurol.2017.185
发表时间：
2018-03
期刊：
Nature reviews. Neurology
影响因子：
--
作者：
Arnold SE;Arvanitakis Z;Macauley-Rambach SL;Koenig AM;Wang HY;Ahima RS;Craft S;Gandy S;Buettner C;Stoeckel LE;Holtzman DM;Nathan DM
通讯作者：
Nathan DM
影响因子：
3
作者：
Andoh M;Koyama R
通讯作者：
Koyama R
小胶质细胞的隔离和培养。
影响因子：
--
作者：
Bohlen CJ;Bennett FC;Bennett ML
通讯作者：
Bennett ML
DOI：
10.1084/jem.20202717
发表时间：
2021-09-06
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Chen Y;Colonna M
通讯作者：
Colonna M
影响因子：
16.2
作者：
Bai, Bing;Wang, Xusheng;Peng, Junmin
通讯作者：
Peng, Junmin