Polymorphic microsatellites in the human bloodfluke, Schistosoma japonicum, identified using a genomic resource.

Polymorphic microsatellites in the human bloodfluke, Schistosoma japonicum, identified using a genomic resource.
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DOI:
10.1186/1756-3305-4-13
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发表时间:
2011-02-07
影响因子:
3.2
通讯作者:
Blair D
Blair D
中科院分区:
医学2区
文献类型:
--
作者:
Xiao N;Remais J;Brindley PJ;Qiu D;Spear R;Lei Y;Blair D

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在中国血吸虫病控制项目已经停止的地区,血吸虫病的重新出现需要发展分子遗传学工具来跟踪基因流动和评估血吸虫种群的遗传多样性。我们确定了许多微卫星位点的基因组草图日本血吸虫使用定义的搜索标准,并选择了一个子集进行进一步分析。从50个位点的初始面板,20个新的微卫星被选择用于最终优化和应用于一个面板的蠕虫从流行地区。所有选定的微卫星,但一个含有简单的三核苷酸重复。检测到中度到高度多态性。等位基因数范围为6 - 14,观察到的杂合性总是>0.6。本文所报道的基因座将有助于对重新出现的病灶中的染色体进行高分辨率的群体遗传学研究。
Re-emergence of schistosomiasis in regions of China where control programs have ceased requires development of molecular-genetic tools to track gene flow and assess genetic diversity of Schistosoma populations. We identified many microsatellite loci in the draft genome of Schistosoma japonicum using defined search criteria and selected a subset for further analysis. From an initial panel of 50 loci, 20 new microsatellites were selected for eventual optimization and application to a panel of worms from endemic areas. All but one of the selected microsatellites contain simple tri-nucleotide repeats. Moderate to high levels of polymorphism were detected. Numbers of alleles ranged from 6 to 14 and observed heterozygosity was always >0.6. The loci reported here will facilitate high resolution population-genetic studies on schistosomes in re-emergent foci.
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