Lipocalin 2 Deficiency Restrains Aging-Related Reshaping of Gut Microbiota Structure and Metabolism.
Lipocalin 2 Deficiency Restrains Aging-Related Reshaping of Gut Microbiota Structure and Metabolism.
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Lipocalin 2缺乏症限制了肠道菌群结构和代谢的衰老相关的重塑。
DOI：
10.3390/biom11091286
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发表时间：
2021-08-28
期刊：
影响因子：
5.5
通讯作者：
Chen X
中科院分区：
文献类型：
--
作者：
Qiu X;Chen C;Chen X
肠道微生物群调节代谢，先天免疫反应和认知功能的年龄相关变化。然而，宿主因素参与调节年龄依赖性肠道微生物结构和肠道炎症在很大程度上是未知的。脂质运载蛋白2（Lcn2）先前已被鉴定为脂肪细胞因子，并且表征为饮食诱导的肥胖和炎症的重要调节剂。先前的研究表明，Lcn2在高脂肪饮食诱导的肠道微生物群重塑和肠道炎症中发挥作用。然而，Lcn2在调节与衰老相关的肠道微生物群重塑中的作用尚不清楚。在此，我们证明了粪便中Lcn2的水平在衰老过程中降低。年龄重塑野生型（WT）小鼠的肠道微生物群组成。有趣的是，Lcn2缺陷减少了Lcn2敲除（LKO）小鼠中的这种衰老效应，导致细菌多样性降低和厚壁菌门与拟杆菌门（F与B）比率增加。具体来说，我们在家族水平上鉴定了16种细菌，这些细菌在老年时在WT和LKO小鼠之间差异丰富。与WT小鼠相比，几种促进健康的细菌（包括产生SCFA的细菌）在老年LKO小鼠中的普遍性显着降低，这表明Lcn 2缺乏将与衰老相关的肠道微生物群落转向不健康的群体，并降低微生物丁酸盐的产生。我们的研究结果提供了一系列证据，证明Lcn2在控制与衰老相关的肠道微生物群组成和代谢物重塑中发挥作用。
Gut microbiota modulate age-associated changes in metabolism, innate immune responses, and cognitive function. However, the involvement of host factors in the regulation of age-dependent gut microbial structure and intestinal inflammation is largely unknown. Lipocalin 2 (Lcn2) has previously been identified as an adipocytokine and characterized as an important regulator of diet-induced obesity and inflammation. Previous studies have shown that Lcn2 plays a role in high fat diet-induced reshaping of gut microbiota and intestinal inflammation. However, the role of Lcn2 in the regulation of aging-related reshaping of gut microbiota is unclear. Herein, we demonstrate that fecal levels of Lcn2 are reduced during aging. Age reshaped gut microbiota composition in wild-type (WT) mice. Interestingly, Lcn2 deficiency diminished this effect of aging in Lcn2 knockout (LKO) mice, leading to decreased bacterial diversity and increased Firmicutes to Bacteroidetes (F to B) ratio. Specifically, we identified 16 bacteria at the family level that were differentially abundant between WT and LKO mice at old age. Several health-promoting bacteria, including SCFA-producing bacteria, were significantly less prevalent in old LKO mice compared to WT mice, indicating that Lcn2 deficiency shifts the aging-related gut microbial community towards an unhealthy population and lowers microbial butyrate production. Our results provide a line of evidence that Lcn2 plays a role in the control of aging-related reshaping of gut microbiota composition and metabolites.
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影响因子：
3.7
作者：
Biagi E;Nylund L;Candela M;Ostan R;Bucci L;Pini E;Nikkïla J;Monti D;Satokari R;Franceschi C;Brigidi P;De Vos W
通讯作者：
De Vos W
影响因子：
7.7
作者：
Guo H;Jin D;Zhang Y;Wright W;Bazuine M;Brockman DA;Bernlohr DA;Chen X
通讯作者：
Chen X
影响因子：
3.7
作者：
Chassaing B;Srinivasan G;Delgado MA;Young AN;Gewirtz AT;Vijay-Kumar M
通讯作者：
Vijay-Kumar M
影响因子：
4.1
作者：
Lu Y;Yao D;Chen C
通讯作者：
Chen C
影响因子：
--
作者：
Biagi, Elena;Candela, Marco;Fairweather-Tait, Susan;Franceschi, Claudio;Brigidi, Patrizia
通讯作者：
Brigidi, Patrizia