Three Proteins (Hpa2, HrpF and XopN) Are Concomitant Type III Translocators in Bacterial Blight Pathogen of Rice
Three Proteins (Hpa2, HrpF and XopN) Are Concomitant Type III Translocators in Bacterial Blight Pathogen of Rice
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三种蛋白（Hpa2、HrpF 和 XopN）是水稻白叶枯病病原体中的伴随 III 型易位蛋白
DOI：
10.3389/fmicb.2020.01601
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发表时间：
2020-07
影响因子：
5.2
通讯作者：
Chen Lei
中科院分区：
文献类型：
--
作者：
Mo Xuyan;Zhang Liyuan;Liu Yan;Wang Xuan;Bai Jiaqi;Lu Kai;Zou Shenshen;Dong Hansong;Chen Lei
III型（T3）蛋白效应子占据了真核生物致病性革兰氏阴性细菌中的大部分毒力决定因子。在感染期间，细菌可能会部署一种称为translocon的纳米机器，将T3效应物递送到宿主细胞中，其中效应物履行其病理功能。T3转位子是由细菌转位子组装而成的，在动物病原菌中被鉴定为一个亲水性蛋白和两个疏水性蛋白，但在植物病原菌中仍不清楚。现在，我们的特点Hpa2，HrpF，和XopN蛋白作为伴随T3易位在水稻白叶枯病病原体通过分析病理后果的单，双，和三重基因敲除或遗传互补。基于这些遗传分析，Hpa2，HrpF，和XopN相应地贡献了46.9%，60.3%和69.8%的细菌毒性的比例对敏感的水稻品种。Hpa2，HrpF，和XopN的毒力表现归因于它们的功能，基本上介导从细菌到水稻细胞易位的PthXo1，细菌T3效应器的转录因子靶向植物基因的特征。平均而言，61%，62%和71%的PthXo1易位相应地由Hpa2，HrpF和XopN提供，而它们以超过95%的程度合作支持PthXo1易位。结果，PthXo1的调控靶基因SWEET11被激活，赋予细菌毒力并诱导水稻的叶枯病。此外，三个易位也进行易位，但只有XopN是高度易位，以抑制水稻防御反应，这表明T3易位子的不同组件部署不同的毒力机制，除了在介导细菌效应子易位的共同功能。
Type III (T3) proteic effectors occupy most of the virulence determinants in eukaryote-pathogenic Gram-negative bacteria. During infection, bacteria may deploy a nanomachinery called translocon to deliver T3 effectors into host cells, wherein the effectors fulfill their pathological functions. T3 translocon is hypothetically assembled by bacterial translocators, which have been identified as one hydrophilic and two hydrophobic proteins in animal-pathogenic bacteria but remain unclear in plant pathogens. Now we characterize Hpa2, HrpF, and XopN proteins as concomitant T3 translocators in rice bacterial blight pathogen by analyzing pathological consequences of single, double, and triple gene knockout or genetic complementation. Based on these genetic analyses, Hpa2, HrpF, and XopN accordingly contribute to 46.9, 60.3, and 69.8% proportions of bacterial virulence on a susceptible rice variety. Virulence performances of Hpa2, HrpF, and XopN were attributed to their functions in essentially mediating from-bacteria-into-rice-cell translocation of PthXo1, the bacterial T3 effector characteristic of transcription factors targeting plant genes. On average, 61, 62, and 71% of PthXo1 translocation are provided correspondingly by Hpa2, HrpF, and XopN, while they cooperate to support PthXo1 translocation at a greater-than-95% extent. As a result, rice disease-susceptibility gene SWEET11, which is the regulatory target of PthXo1, is activated to confer bacterial virulence and induce the leaf blight disease in rice. Furthermore, the three translocators also undergo translocation, but only XopN is highly translocated to suppress rice defense responses, suggesting that different components of a T3 translocon deploy distinct virulence mechanisms in addition to the common function in mediating bacterial effector translocation.
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影响因子：
5.4
作者：
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通讯作者：
J. Santi-Rocca;N. Blanchard
影响因子：
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作者：
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通讯作者：
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作者：
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作者：
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影响因子：
27.4
作者：
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