Brain-derived neurotrophic factor in arterial baroreceptor pathways: implications for activity-dependent plasticity at baroafferent synapses.
Brain-derived neurotrophic factor in arterial baroreceptor pathways: implications for activity-dependent plasticity at baroafferent synapses.
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DOI：
10.1111/j.1471-4159.2008.05781.x
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发表时间：
2009-01
影响因子：
4.7
通讯作者：
Balkowiec, Agnieszka
中科院分区：
文献类型：
--
作者：
Martin, Jessica L.;Jenkins, Victoria K.;Hsieh, Hui-ya;Balkowiec, Agnieszka
关键词：
动脉压力感受器反射在整个个体发生过程中的功能特征，包括围生期反射增益的调节和高血压期间的成人重置。然而，这些功能变化背后的细胞机制尚不完全清楚。在这里，我们提供的证据表明，脑源性神经营养因子（BDNF）是一种在活动依赖性神经元可塑性中发挥重要作用的神经营养因子，在发育和成年大鼠压力感受器传入神经中大量表达。对BDNF的免疫反应存在于结节神经节（NG）的压力传入神经元的细胞体、它们在孤立束的中央突起和脑干下部孤束核（NTS）的终末样结构中。使用ELISA原位结合电场刺激，我们发现天然BDNF从培养的新生NG神经元中释放，以响应模拟体内压力感受器传入活动的模式。特别是，压力感受器放电的高频爆发模式，已知会引起压力感受器突触的可塑性变化，在释放BDNF方面明显比相同平均频率的强直性模式更有效。总之，我们的研究表明，一级压力感受器神经元表达的BDNF可能是动脉压力感受器通路一级突触发育和发育后修饰的介质。
Functional characteristics of the arterial baroreceptor reflex change throughout ontogenesis, including perinatal adjustments of the reflex gain and adult resetting during hypertension. However, the cellular mechanisms that underlie these functional changes are not completely understood. Here, we provide evidence that brain-derived neurotrophic factor (BDNF), a neurotrophin with a well-established role in activity-dependent neuronal plasticity, is abundantly expressed in vivo by a large subset of developing and adult rat baroreceptor afferents. Immunoreactivity to BDNF is present in the cell bodies of baroafferent neurons in the nodose ganglion (NG), their central projections in the solitary tract, and terminal-like structures in the lower brainstem nucleus tractus solitarius (NTS). Using ELISA in situ combined with electrical field stimulation, we show that native BDNF is released from cultured newborn NG neurons in response to patterns that mimic the in vivo activity of baroreceptor afferents. In particular, high-frequency bursting patterns of baroreceptor firing, which are known to evoke plastic changes at baroreceptor synapses, are significantly more effective at releasing BDNF than tonic patterns of the same average frequency. Together, our study indicates that BDNF expressed by first-order baroreceptor neurons is a likely mediator of both developmental and post-developmental modifications at first-order synapses in arterial baroreceptor pathways.
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影响因子：
2.7
作者：
Bennett, HL;Gustafsson, JÅ;Keast, JR
通讯作者：
Keast, JR
DOI：
10.1046/j.1440-1681.2002.03666.x
发表时间：
2002-05-01
影响因子：
2.9
作者：
Gordon, FJ;Sved, AF
通讯作者：
Sved, AF
影响因子：
5.3
作者：
Balkowiec, A;Katz, DM
通讯作者：
Katz, DM
影响因子：
5.3
作者：
Doan, TN;Stephans, K;Kunze, DL
通讯作者：
Kunze, DL
影响因子：
3.3
作者：
Arsenault, J;Moreau-Bussière, F;Praud, JP
通讯作者：
Praud, JP