Genome duplication improves rice root resistance to salt stress.
Genome duplication improves rice root resistance to salt stress.
复制标题
基因组复制提高了水稻根部对盐胁迫的抵抗力
DOI：
10.1186/s12284-014-0015-4
复制
发表时间：
2014
期刊：
影响因子：
--
通讯作者：
He Y
中科院分区：
文献类型：
--
作者：
Tu Y;Jiang A;Gan L;Hossain M;Zhang J;Peng B;Xiong Y;Song Z;Cai D;Xu W;Zhang J;He Y
盐胁迫是限制作物生产力的一种胁迫环境因子，而根系是植物与各种非生物胁迫之间的主要界面。水稻是一种盐敏感作物，其多倍体在抗盐性方面具有优势。本研究的目的是探讨基因组重复对水稻根系抗盐stress. ResultsTwo二倍体水稻（HN 2026 -2x和日本晴-2x）和相应的四倍体水稻（HN 2026 -4x和日本晴-4x）的影响培养在半强度Murashige和Skoog培养基与150 mM NaCl为3和5天。测定了水稻根尖脯氨酸、可溶性糖、丙二醛（MDA）、Na+含量、H+（质子）通量的积累以及根的显微和超微结构。结果表明，四倍体水稻根系生长抑制程度较二倍体水稻低，脯氨酸含量较高，丙二醛含量较二倍体水稻低，正常表皮细胞的比例较高。此外，四倍体水稻皮层和周皮层细胞之间存在保护间隙。接下来，超微结构分析表明，基因组复制提高膜，细胞器和细胞核的稳定性。此外，Na+在四倍体水稻根显着下降，而根尖H+流出在四倍体rice.ConclusionsOur结果表明，基因组复制提高根耐盐胁迫，增强质子运输到根表面可能发挥作用，减少Na+进入根。
BackgroundSalinity is a stressful environmental factor that limits the productivity of crop plants, and roots form the major interface between plants and various abiotic stresses. Rice is a salt-sensitive crop and its polyploid shows advantages in terms of stress resistance. The objective of this study was to investigate the effects of genome duplication on rice root resistance to salt stress.ResultsBoth diploid rice (HN2026-2x and Nipponbare-2x) and their corresponding tetraploid rice (HN2026-4x and Nipponbare-4x) were cultured in half-strength Murashige and Skoog medium with 150 mM NaCl for 3 and 5 days. Accumulations of proline, soluble sugar, malondialdehyde (MDA), Na+content, H+(proton) flux at root tips, and the microstructure and ultrastructure in rice roots were examined. We found that tetraploid rice showed less root growth inhibition, accumulated higher proline content and lower MDA content, and exhibited a higher frequency of normal epidermal cells than diploid rice. In addition, a protective gap appeared between the cortex and pericycle cells in tetraploid rice. Next, ultrastructural analysis showed that genome duplication improved membrane, organelle, and nuclei stability. Furthermore, Na+in tetraploid rice roots significantly decreased while root tip H+efflux in tetraploid rice significantly increased.ConclusionsOur results suggest that genome duplication improves root resistance to salt stress, and that enhanced proton transport to the root surface may play a role in reducing Na+entrance into the roots.
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DOI：
10.3923/ijb.2011.73.81
发表时间：
2011-01-01
期刊：
International Journal of Botany
影响因子：
--
作者：
Amirjani, Mohammad Reza
通讯作者：
Amirjani, Mohammad Reza
影响因子：
6.9
作者：
Colmer, TD;Flowers, TJ;Munns, R
通讯作者：
Munns, R
影响因子：
6.9
作者：
Barrowclough, DE;Peterson, CA;Steudle, E
通讯作者：
Steudle, E
影响因子：
1.5
作者：
Al-Khayri, JM;Al-Bahrany, AM
通讯作者：
Al-Bahrany, AM
影响因子：
3
作者：
Azevedo, Herlander;Amorim-Silva, Vitor;Tavares, Rui M.
通讯作者：
Tavares, Rui M.