Identification of highly-adhesive gut Lactobacillus strains in zebrafish (Danio rerio) by partial rpoB gene sequence analysis
Identification of highly-adhesive gut Lactobacillus strains in zebrafish (Danio rerio) by partial rpoB gene sequence analysis
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通过部分 rpoB 基因序列分析鉴定斑马鱼（Danio rerio）高粘附性肠道乳酸菌菌株
DOI：
10.1016/j.aquaculture.2012.10.018
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发表时间：
2012-12
期刊：
影响因子：
4.5
通讯作者：
Ringo, Einar
中科院分区：
文献类型：
--
作者：
Zhou, Zhigang;Wang, Wenwen;Liu, Wenshu;Gatlin, Delbert M., III;Zhang, Yuting;Yao, Bin;Ringo, Einar
利用rpoB基因片段和变性梯度凝胶电泳（DGGE）技术对斑马鱼肠道高粘附性乳酸杆菌进行鉴定。在循环水养殖系统（20尾/池，6池/处理）中，进行为期4周的饲养试验（每天2次至视觉饱足），试验饲料为对照饲料和添加等量益生乳酸菌的益生饲料（6.0× 107个/g对照饲料）。分别在实验开始和第1、2、3、4周结束时，于第二次喂食后1h采集肠道粘附菌。结果表明，在不同的饲养阶段，肠道优势粘附乳酸杆菌的组成在菌株丰富度和相对丰度上变化较大。2028、布氏乳杆菌BU 222、植物乳杆菌植物亚种（Lactobacillus plantarum subsp. plantarum JCM 1149 T、鼠李糖乳杆菌（Lactobacillus rhamnosus）CICC 6141和L.基于DGGE和克隆文库分析两者，将rhamnosus JCM 20300鉴定为第二周样品的高粘附肠道菌株。以嗜水气单胞菌NJ-1为攻毒剂，比较了高、低粘附力菌株JCM 1149 T和嗜酸乳杆菌JCM 1132 T（10 ~（-6）cells/g饲料）对斑马鱼的保护作用。菌株JCM 1149 T在体外对NJ-1菌株的抗菌活性明显高于菌株JCM 1132 T。这加强了这样的判断，即胃肠道（GI）粘连是一个有利的性质，饮食益生菌作为生物控制剂在水产养殖。结论：以斑马鱼为模式鱼，基于部分rpoB基因-PCR的DGGE分析可有效筛选和定性监测肠道益生乳酸杆菌的高粘附性菌株。
The objective of the present study was to identify the highly-adhesive gut Lactobacillus strains in zebrafish (Danio rerio) by employing partial rpoB gene and denaturing gradient gel electrophoresis (DGGE). A four-week feeding trial (twice a day to visual satiation) with two diets, a control diet and a probiotic diet supplemented with ten probiotic Lactobacillus strains at equal amounts (6.0×107cells/g control diet), was conducted in a recirculation aquaculture system (20 fish/tank, 6 tanks/treatment). Adhesive gut bacteria were sampled 1h later after the second feeding at the experimental start and the end of 1, 2, 3, and 4weeks, respectively. The results showed that the composition of predominant adhesive gut lactobacilli varied greatly in strain richness and relative abundance during the feeding period. Lactobacillus brevis CGMCC 1.2028, Lactobacillus buchneri BU 222, Lactobacillus plantarum subsp. plantarum JCM 1149T, Lactobacillus rhamnosus CICC 6141, and L. rhamnosus JCM 20300 were identified as the highly-adhesive gut strains of the second week samples based on both DGGE and clone library analysis. The protective effects of highly- and less-adhesive strains JCM 1149Tand Lactobacillus acidophilus JCM 1132T(106–8cells/g diet) on zebrafish were compared in an immersion challenge with Aeromonas hydrophila NJ-1 after 14-day-feeding. Strain JCM 1149Tperformed obviously more efficiently than strain JCM 1132Tagainst strain NJ-1 in vitro. This strengthened the judgment that gastrointestinal (GI) tract adhesion is a favorable property of dietary probiotics as biocontrol agents in aquaculture. It was concluded that DGGE analysis based on partial rpoB gene-PCR was efficient to screen and qualitatively monitor the highly-adhesive gut strains from potential probiotic lactobacilli as zebrafish a model fish species.
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影响因子：
--
作者：
Ouwehand, Arthur C.;Salminen, Seppo
通讯作者：
Salminen, Seppo
DOI：
--
发表时间：
2004
期刊：
--
影响因子：
--
作者：
A. Galindo
通讯作者：
A. Galindo
DOI：
--
发表时间：
2007
期刊：
Chinese Journal of Animal Nutrition
影响因子：
--
作者：
Zhi-gang Zhou
通讯作者：
Zhi-gang Zhou
影响因子：
4
作者：
Huber, I;Spanggaard, B;Gram, L
通讯作者：
Gram, L
影响因子：
3.3
作者：
M. Rinkinen;E. Westermarck;S. Salminen;A. Ouwehand
通讯作者：
M. Rinkinen;E. Westermarck;S. Salminen;A. Ouwehand