Induction of nitric oxide synthase mRNA in coronary resistance arteries isolated from exercise-trained pigs.
Induction of nitric oxide synthase mRNA in coronary resistance arteries isolated from exercise-trained pigs.
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从经过运动训练的猪身上分离出的冠状动脉阻力动脉中一氧化氮合酶 mRNA 的诱导。
DOI：
10.1152/ajpheart.1997.273.6.h2575
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Price,EM
中科院分区：
文献类型：
--
作者：
Woodman,CR;Muller,JM;Laughlin,MH;Price,EM
本研究的目的是开发一种方法，通过它可以测量内皮细胞一氧化氮合酶（ecNOS）mRNA的表达在单个冠状动脉阻力动脉和测试的假设，ecNOS基因表达上调运动训练。将尤卡坦小型猪随机分为运动训练组（ET;n= 5）和安静组（Sed;n= 4），为期16周。解剖单个冠状动脉阻力动脉（50-100 μm），在液氮中冷冻，并在LiCl缓冲液中匀浆。从每个容器中分离mRNA，并使用逆转录酶（RT）-聚合酶链反应（PCR）通过与甘油醛3-磷酸脱氢酶（GAPDH）共扩增ecNOS标准化的ecNOS基因表达进行评估。从ET猪分离的冠状动脉阻力动脉中ecNOS与GAPDH扩增子的比率显著高于Sed对照。在这些数据的基础上，可以得出结论，RT-PCR可以用于单冠状动脉阻力动脉，以评估细胞特异性mRNA的表达和ecNOS基因的表达上调运动训练在猪冠状动脉阻力动脉。
The purpose of this study was to develop a method by which endothelial cell nitric oxide synthase (ecNOS) mRNA expression could be measured in single coronary resistance arteries and to test the hypothesis that ecNOS gene expression is upregulated by exercise training. Yucatan miniature swine were randomly assigned to exercise-trained (ET;n= 5) or sedentary (Sed;n= 4) groups for 16 wk. Individual coronary resistance arteries (50–100 μm) were dissected, frozen in liquid nitrogen, and homogenized in a LiCl buffer. mRNA was isolated from each vessel, and ecNOS gene expression was assessed using reverse transcriptase (RT)-polymerase chain reaction (PCR) standardized by coamplifying ecNOS with glyceraldehyde 3-phosphate dehydrogenase (GAPDH). The ecNOS-to-GAPDH amplicon ratio was significantly greater in coronary resistance arteries isolated from ET pigs than in Sed controls. On the basis of these data, it is concluded that RT-PCR can be used on single coronary resistance arteries to assess cell-specific mRNA expression and that ecNOS gene expression is upregulated by exercise training in porcine coronary resistance arteries.
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影响因子：
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影响因子：
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影响因子：
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DOI：
10.1152/jappl.1985.58.2.468
发表时间：
1985
期刊：
Journal of applied physiology (Bethesda, Md. : 1985)
影响因子：
--
作者：
Laughlin,MH
通讯作者：
Laughlin,MH