Protective Roles of γδ T Cells and Interleukin-15 in Escherichia coli Infection in Mice
Protective Roles of γδ T Cells and Interleukin-15 in Escherichia coli Infection in Mice
复制标题
γδ T 细胞和白细胞介素 15 在小鼠大肠杆菌感染中的保护作用
作者：
M. Takano;H. Nishimura;Y. Kimura;Y. Mokuno;J. Washizu;S. Itohara;Y. Nimura;Y. Yoshikai
腹腔注射（i. p.）在脂多糖（LPS）应答的C3 H/HeN小鼠中感染大肠杆菌，但在LPS低应答的C3 H/HeJ小鼠中不感染。γδ T细胞优先表达恒定的Vγ6和Vδ1链，并在LPS存在下增殖以产生大量γ干扰素。T细胞受体δ基因突变导致γδ T细胞耗竭的小鼠对E.通过细菌生长评估大肠杆菌。C_3H/HeN小鼠感染E.大肠杆菌中IL-15 mRNA的表达水平高于C3 H/HeJ小鼠。给予抗IL-15单克隆抗体可部分抑制C3 H/HeN小鼠E.大肠杆菌感染，降低了宿主对感染的防御能力。这些结果表明，LPS刺激的γδ T细胞在宿主防御E. IL-15可能通过增加γδ T细胞的数量而部分参与了保护作用。
ABSTRACT The number of γδ T cells in the peritoneal cavity was increased after an intraperitoneal (i.p.) infection with Escherichia coli in lipopolysaccharide (LPS)-responsive C3H/HeN mice but not in LPS-hyporesponsive C3H/HeJ mice. The γδ T cells preferentially expressed invariant Vγ6 and Vδ1 chains and proliferated to produce a large amount of gamma interferon in the presence of LPS. Mice depleted of γδ T cells by T-cell receptor δ gene mutation showed impaired resistance against E. coli as assessed by bacterial growth. Macrophages from C3H/HeN mice infected with E. coli expressed higher levels of interleukin-15 (IL-15) mRNA than those from the infected C3H/HeJ mice. Administration of anti-IL-15 monoclonal antibody inhibited, albeit partially, the appearance of γδ T cells in C3H/HeN mice after E. coli infection and diminished the host defense against the infection. These results suggest that LPS-stimulated γδ T cells play an important role in the host defense against E. coli infection and that IL-15 may be partly involved in the protection via an increase in the γδ T cells.
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DOI：
10.1073/pnas.86.2.631
发表时间：
1989-01-01
影响因子：
11.1
作者：
ITO, K;BONNEVILLE, M;TONEGAWA, S
通讯作者：
TONEGAWA, S
CD4 αβ T 细胞和 γ δ T 细胞对结核分枝杆菌的反应。
DOI：
--
发表时间：
1995
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Tsukaguchi,K;Balaji,KN;Boom,WH
通讯作者：
Boom,WH
疱疹病毒特异性 TCR-gamma delta 细胞具有独特的抗原识别能力。
DOI：
--
发表时间：
1994
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Sciammas,R;Johnson,RM;Sperling,AI;Brady,W;Linsley,PS;Spear,PG;Fitch,FW;Bluestone,JA
通讯作者：
Bluestone,JA
细菌感染期间 γ δ T 细胞受体不变子集的反应。
DOI：
--
发表时间：
1996
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Roark,CE;Vollmer,MK;Campbell,PA;Born,WK;O'Brien,RL
通讯作者：
O'Brien,RL
DOI：
10.1073/pnas.93.21.11774
发表时间：
1996-10-15
影响因子：
11.1
作者：
Roberts, SJ;Smith, AL;Hayday, AC
通讯作者：
Hayday, AC