Imaging-based spectrometer-less optofluidic biosensors based on dielectric metasurfaces for detecting extracellular vesicles.
Imaging-based spectrometer-less optofluidic biosensors based on dielectric metasurfaces for detecting extracellular vesicles.
复制标题
基于介电超表面检测细胞外囊泡的成像无光谱光流体生物传感器。
DOI：
10.1038/s41467-021-23257-y
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发表时间：
2021-05-31
影响因子：
16.6
通讯作者：
Altug H
中科院分区：
文献类型：
--
作者：
Jahani Y;Arvelo ER;Yesilkoy F;Koshelev K;Cianciaruso C;De Palma M;Kivshar Y;Altug H
生物传感器是公共、全球和个性化医疗保健不可或缺的工具，因为它们提供的测试可用于从早期疾病检测和治疗监测到预防大流行病。我们介绍了单波长成像生物传感器，能够重建生物标志物动态使用先进的数据处理技术的基础上的最佳线性估计引起的光谱偏移信息。我们的方法实现了上级灵敏度没有波长扫描或光谱仪器。我们设计了双原子介电超颖表面，支持连续体中的束缚态，通过面内对称性破缺实现高质量的近场共振。大面积超表面芯片被配置为微阵列，并与成像平台上的微流体集成，用于实时检测包含外泌体的乳腺癌细胞外囊泡。该光流体系统具有高传感性能，具有近70 1/RIU的优值，能够检测平均0.41纳米颗粒/μm2，并实时测量从低至204飞摩尔溶液的细胞外囊泡结合。我们的生物传感器提供了光谱方法的鲁棒性，同时用单波长光源和互补金属氧化物半导体相机取代了复杂的仪器，为医疗点诊断的小型化设备铺平了道路。作者在双原子介电超颖表面的连续体中设计了一种束缚态，允许高质量的共振与可访问的增强场。超表面微阵列与微流体集成在成像平台上，用于基于重建光谱位移信息实时检测生物样品。
Biosensors are indispensable tools for public, global, and personalized healthcare as they provide tests that can be used from early disease detection and treatment monitoring to preventing pandemics. We introduce single-wavelength imaging biosensors capable of reconstructing spectral shift information induced by biomarkers dynamically using an advanced data processing technique based on an optimal linear estimator. Our method achieves superior sensitivity without wavelength scanning or spectroscopy instruments. We engineered diatomic dielectric metasurfaces supporting bound states in the continuum that allows high-quality resonances with accessible near-fields by in-plane symmetry breaking. The large-area metasurface chips are configured as microarrays and integrated with microfluidics on an imaging platform for real-time detection of breast cancer extracellular vesicles encompassing exosomes. The optofluidic system has high sensing performance with nearly 70 1/RIU figure-of-merit enabling detection of on average 0.41 nanoparticle/µm2 and real-time measurements of extracellular vesicles binding from down to 204 femtomolar solutions. Our biosensors provide the robustness of spectrometric approaches while substituting complex instrumentation with a single-wavelength light source and a complementary-metal-oxide-semiconductor camera, paving the way toward miniaturized devices for point-of-care diagnostics. The authors engineer a type of bound states in the continuum in diatomic dielectric metasurfaces, allowing for high-quality resonances with accessible enhanced fields. Metasurface microarrays are integrated with microfluidics on an imaging platform for real-time detection of biosamples, based on reconstructing spectral shift information.
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影响因子：
7.3
作者：
Andreu Z;Yáñez-Mó M
通讯作者：
Yáñez-Mó M
影响因子：
10.8
作者：
Gholipour, Behrad;Piccinotti, Davide;Zheludev, Nikolay, I
通讯作者：
Zheludev, Nikolay, I
影响因子：
19.4
作者：
Cetin, Arif E.;Coskun, Ahmet F.;Altug, Hatice
通讯作者：
Altug, Hatice
影响因子：
7
作者：
Doiron, Brock;Mota, Monica;Maier, Stefan A.
通讯作者：
Maier, Stefan A.
影响因子：
8.6
作者：
Koshelev, Kirill;Lepeshov, Sergey;Kivshar, Yuri
通讯作者：
Kivshar, Yuri