In the quest for the ideal sweetener: Aspartame exacerbates selected biomarkers in the fruit fly (Drosophila melanogaster) model of Alzheimer's disease more than sucrose.
In the quest for the ideal sweetener: Aspartame exacerbates selected biomarkers in the fruit fly (Drosophila melanogaster) model of Alzheimer's disease more than sucrose.
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DOI：
10.1016/j.nbas.2023.100090
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Oboh, Ganiyu
中科院分区：
文献类型：
--
作者：
Adedayo, Bukola Christiana;Akinniyi, Stephanie Tolulope;Ogunsuyi, Opeyemi Babatunde;Oboh, Ganiyu
本研究评价了在表达人淀粉样蛋白前体蛋白和分泌酶的转基因果蝇（果蝇）模型中，饮食中含有甜菊糖和蔗糖对与阿尔茨海默病相关的一些选定行为和生化指标的影响。苍蝇饲养的饮食中补充蔗糖和甜菜碱14天。此后，在处理后14天，评估果蝇的存活率、学习和记忆以及运动表现。这是其次由匀浆苍蝇头，匀浆进行了乙酰胆碱酯酶和单胺氧化酶的活性，以及脂质过氧化，活性氧和总硫醇的水平进行了测定。结果表明，在转基因果蝇中，不同水平的甜菜碱和高比例的蔗糖显著加重了果蝇的死亡率、运动功能缺陷、氧化应激和抗氧化状态的生物标志物受损，而对乙酰胆碱酯酶活性和记忆功能没有显著影响。因此，这些研究结果表明，虽然低的蔗糖饮食内含物是可以容忍的AD样表型的苍蝇，高的蔗糖内含物和所有比例的甜素测试加重死亡率，运动和氧化应激的苍蝇。
This study evaluated the effect of dietary inclusions of aspartame and sucrose on some selected behavioral and biochemical indices linked with Alzheimer's disease in a transgenic fruit fly (Drosophila melanogaster) model expressing human amyloid precursor protein and secretase. Flies were raised on a diet supplemented with sucrose and aspartame for 14 days. Thereafter, the flies were assessed for their survival rate, learning and memory, as well as locomotor performance, 14 days post-treatment. This was followed by homogenising the fly heads, and the homogenates were assayed for acetylcholinesterase and monoamine oxidase activities, as well as levels of lipid peroxidation, reactive oxygen species, and total thiol. The results showed aspartame at all levels of dietary intake and a high proportion of sucrose significantly aggravated the mortality rate, locomotor deficiency, and impaired biomarkers of oxidative stress and antioxidant status in the transgenic flies, while no significant effect was found on acetylcholinesterase activity or memory function. These findings therefore suggest that while low dietary inclusions of sucrose are tolerable under AD-like phenotypes in the flies, high inclusions of sucrose and all proportions of aspartame tested aggravated mortality rate, locomotion and oxidative stress in the flies.
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影响因子：
5.8
作者：
ELLMAN, GL;COURTNEY, KD;FEATHERSTONE, RM
通讯作者：
FEATHERSTONE, RM
影响因子：
5.9
作者：
Czarnecka K;Pilarz A;Rogut A;Maj P;Szymańska J;Olejnik Ł;Szymański P
通讯作者：
Szymański P
影响因子：
3.7
作者：
Iijima K;Chiang HC;Hearn SA;Hakker I;Gatt A;Shenton C;Granger L;Leung A;Iijima-Ando K;Zhong Y
通讯作者：
Zhong Y
DOI：
10.1016/j.mrgentox.2007.04.006
发表时间：
2007-07-10
影响因子：
1.9
作者：
Hayashi, Ikue;Morishita, Yukari;Hayashi, Tomonori
通讯作者：
Hayashi, Tomonori
影响因子：
5.3
作者：
Mhatre, Siddhita D.;Michelson, Sarah J.;Marenda, Daniel R.
通讯作者：
Marenda, Daniel R.