Interfacial polarization of in vivo rat sciatic nerve with crush injury studied via broadband dielectric spectroscopy.
Interfacial polarization of in vivo rat sciatic nerve with crush injury studied via broadband dielectric spectroscopy.
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DOI：
10.1371/journal.pone.0252589
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发表时间：
2021
期刊：
影响因子：
3.7
通讯作者：
Aoyama T
中科院分区：
文献类型：
--
作者：
Otagiri R;Kawai H;Takatsuka M;Shinyashiki N;Ito A;Ikeguchi R;Aoyama T
电刺激是损伤的周围神经的伸长驱动再生的候选者之一。不同的器官和组织具有固有的细胞结构和大小。这导致界面极化频率的组织特异性电特性的变化。虽然神经组织具有膜电位，但是这些组织内部在来自外部的电刺激之后的电反应仍然未被探索。此外，受损神经的病理生理反应尚不清楚。在这里，我们研究了电反应的损伤和非损伤大鼠坐骨神经通过宽带介电谱。在造成损伤后6天，使用6只12周龄雄性刘易斯大鼠的挤压损伤和未损伤的坐骨神经。两边的神经（有无损伤）暴露，在室温下进行阻抗测量（约25 ℃）在频率范围从100 mHz到5.5 MHz和电位范围从0.100到1.00 V。所测量的界面极化可能起源于在3.2 kHz和1.6 kHz之间的频率下通过神经膜周围的离子运输的极化MHz.损伤神经的极化强度小于未损伤神经。然而，损伤和非损伤神经之间的极化差异可能是由炎症和水肿引起的。界面极化的合适频率范围可以预期是损伤的周围神经的电刺激的关键。
Electrical stimulation is one of the candidates for elongation-driven regeneration of damaged peripheral nerves. Different organs and tissues have an inherent cell structure and size. This leads to variation in the tissue-specific electrical properties of the frequency of interfacial polarization. Although nervous tissues have a membrane potential, the electrical reaction inside these tissues following electrical stimulation from outside remains unexplored. Furthermore, the pathophysiological reaction of an injured nerve is unclear. Here, we investigated the electrical reaction of injured and non-injured rat sciatic nerves via broadband dielectric spectroscopy. Crush injured and non-injured sciatic nerves of six 12-week-old male Lewis rats were used, 6 days after infliction of the injury. Both sides of the nerves (with and without injury) were exposed, and impedance measurements were performed at room temperature (approximately 25°C) at frequencies ranging from 100 mHz to 5.5 MHz and electric potential ranging from 0.100 to 1.00 V. The measured interfacial polarization potentially originated from the polarization by ion transport around nerve membranes at frequencies between 3.2 kHz and 1.6 MHz. The polarization strength of the injured nerves was smaller than that of non-injured nerves. However, the difference in polarization between injured and non-injured nerves might be caused by inflammation and edema. The suitable frequency range of the interfacial polarization can be expected to be critical for electrical stimulation of injured peripheral nerves.
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影响因子：
16.6
作者：
Ravagli E;Mastitskaya S;Thompson N;Iacoviello F;Shearing PR;Perkins J;Gourine AV;Aristovich K;Holder D
通讯作者：
Holder D
影响因子：
20.6
作者：
SCHWAN, HP;FOSTER, KR
通讯作者：
FOSTER, KR
影响因子：
--
作者：
Grinsell D;Keating CP
通讯作者：
Keating CP
影响因子：
0.6
作者：
Byrne, Kelly;Tsui, Ban C. H.
通讯作者：
Tsui, Ban C. H.
DOI：
10.1016/j.jphysparis.2017.05.003
发表时间：
2016-11-01
期刊：
Journal of physiology, Paris
影响因子：
--
作者：
Fouchard, Alexandre;Coizet, Veronique;David, Olivier
通讯作者：
David, Olivier