Ultrastructural characterization of microlipophagy induced by the interaction of vacuoles and lipid bodies around generative and sperm cells in Arabidopsis pollen.
Ultrastructural characterization of microlipophagy induced by the interaction of vacuoles and lipid bodies around generative and sperm cells in Arabidopsis pollen.
复制标题
DOI：
10.1007/s00709-020-01557-2
复制
发表时间：
2021-01
期刊：
影响因子：
2.9
通讯作者：
Nagata N
中科院分区：
文献类型：
--
作者：
Akita K;Takagi T;Kobayashi K;Kuchitsu K;Kuroiwa T;Nagata N
在花粉成熟过程中，各种细胞器在雄配子体发育过程中改变其分布和功能。利用连续切片扫描电镜和常规透射电镜分析了拟南芥花粉中参与噬脂作用的脂体和液泡的行为。在二细胞花粉时期，营养细胞中的脂体排列在生殖细胞表面。然后，脂质体紧密地附着、吸入并降解脂质体，并最终占据脂质体的空间。在脂质体的全部内容物转移之前，脂质开始降解。在三细胞期，精细胞周围不再有脂质体，而是空泡。脂质体的降解在形态学上被认为是微自噬。atg 2 -1拟南芥突变体缺乏一个自噬相关基因（ATG）。在该突变体中，精子细胞周围的液泡比野生型花粉稀疏。ATG 2的缺乏可能阻止或减缓脂质降解，尽管它不阻止细胞器之间的接触。这些结果表明，在拟南芥花粉发育的microlipophagy参与。本文的在线版本（10.1007/s 00709 -020-01557-2）包含补充材料，可供授权用户使用。
During pollen maturation, various organelles change their distribution and function during development as male gametophytes. We analyzed the behavior of lipid bodies and vacuoles involved in lipophagy in Arabidopsis pollen using serial section SEM and conventional TEM. At the bicellular pollen stage, lipid bodies in the vegetative cells lined up at the surface of the generative cell. Vacuoles then tightly attached, drew in, and degraded the lipid bodies and eventually occupied the space of the lipid bodies. Degradation of lipid began before transfer of the entire contents of the lipid body. At the tricellular stage, vacuoles instead of lipid bodies surrounded the sperm cells. The degradation of lipid bodies is morphologically considered microautophagy. The atg2-1 Arabidopsis mutant is deficient in one autophagy-related gene (ATG). In this mutant, the assembly of vacuoles around sperm cells was sparser than that in wild-type pollen. The deficiency of ATG2 likely prevents or slows lipid degradation, although it does not prevent contact between organelles. These results demonstrate the involvement of microlipophagy in the pollen development of Arabidopsis. The online version of this article (10.1007/s00709-020-01557-2) contains supplementary material, which is available to authorized users.
登录
查看更多内容
DOI：
10.2183/pjab.86.455
发表时间：
2010
期刊：
Proceedings of the Japan Academy. Series B, Physical and biological sciences
影响因子：
--
作者：
Kuroiwa T
通讯作者：
Kuroiwa T
影响因子：
5.8
作者：
Arganda-Carreras, Ignacio;Kaynig, Verena;Seung, H. Sebastian
通讯作者：
Seung, H. Sebastian
影响因子：
5.5
作者：
Gao Q;Goodman JM
通讯作者：
Goodman JM
影响因子：
6.2
作者：
Kang, Sangwoo;Shin, Kwang Deok;Chung, Taijoon
通讯作者：
Chung, Taijoon
影响因子：
2.8
作者：
Nagata, Noriko
通讯作者：
Nagata, Noriko