Intranasal immunization with inactivated SARS-CoV (SARS-associated coronavirus) induced local and serum antibodies in mice.
Intranasal immunization with inactivated SARS-CoV (SARS-associated coronavirus) induced local and serum antibodies in mice.
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DOI：
10.1016/j.vaccine.2004.07.031
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发表时间：
2005-01-04
期刊：
影响因子：
5.5
通讯作者：
Wen YM
中科院分区：
文献类型：
--
作者：
Qu D;Zheng B;Yao X;Guan Y;Yuan ZH;Zhong NS;Lu LW;Xie JP;Wen YM
用1:2000甲醛对SARS-CoV（严重急性呼吸综合征相关冠状病毒）菌株GZ50进行部分纯化灭活。在细胞培养中，灭活病毒阻断活病毒的复制，使活病毒的TCID5.0降低103.6 ~ 104.6倍。灭活的GZ50单独或经聚乙二醇（PEG）沉淀或与CpG或CTB作为佐剂后用于鼻内免疫小鼠。血清中和抗体滴度高达1:640。用佐剂或PEG沉淀GZ50免疫小鼠，用免疫荧光法检测气管-肺洗液中特异性IgA。仅用灭活GZ50免疫小鼠，血清中虽检测到抗体，但未检测到抗sars - iga。佐剂在鼻内灭活免疫中的作用。讨论了SARS-CoV。
SARS-CoV (severe acute respiratory syndrome-associated coronavirus) strain GZ50 was partially purified and inactivated with 1:2000 formaldehyde. In cell culture the inactivated virus blocked the replication of live virus by decreasing the TCID5.0 of the live virus 103.6 to 104.6 times. Inactivated GZ50 was used to immunize mice intranasally either alone, or after precipitation with polyethylene glycol (PEG), or with CpG, or CTB as an adjuvant. The titer of serum neutralizing antibodies was up to 1:640. In mice immunized with adjuvants or PEG precipitated GZ50, specific IgA was detected in tracheal-lung wash fluid by immunofluorescence. Though serum antibodies were detected, no anti-SARS-IgA could be detected in mice immunized only with inactivated GZ50. The roles of adjuvants in intranasal immunization with inactivated. SARS-CoV is discussed.
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影响因子：
64.8
作者：
Li W;Moore MJ;Vasilieva N;Sui J;Wong SK;Berne MA;Somasundaran M;Sullivan JL;Luzuriaga K;Greenough TC;Choe H;Farzan M
通讯作者：
Farzan M
DOI：
10.1016/s0140-6736(03)13077-2
发表时间：
2003-04-19
期刊：
Lancet (London, England)
影响因子：
--
作者：
Peiris JS;Lai ST;Poon LL;Guan Y;Yam LY;Lim W;Nicholls J;Yee WK;Yan WW;Cheung MT;Cheng VC;Chan KH;Tsang DN;Yung RW;Ng TK;Yuen KY;SARS study group
通讯作者：
SARS study group
影响因子：
6.4
作者：
Lowell, GH;Kaminski, RW;Birx, DL
通讯作者：
Birx, DL
DOI：
10.1016/s0140-6736(03)13413-7
发表时间：
2003-05-24
期刊：
Lancet (London, England)
影响因子：
--
作者：
Nicholls JM;Poon LL;Lee KC;Ng WF;Lai ST;Leung CY;Chu CM;Hui PK;Mak KL;Lim W;Yan KW;Chan KH;Tsang NC;Guan Y;Yuen KY;Peiris JS
通讯作者：
Peiris JS
DOI：
10.1016/s0140-6736(03)13414-9
发表时间：
2003-05-24
期刊：
Lancet (London, England)
影响因子：
--
作者：
Ruan YJ;Wei CL;Ee AL;Vega VB;Thoreau H;Su ST;Chia JM;Ng P;Chiu KP;Lim L;Zhang T;Peng CK;Lin EO;Lee NM;Yee SL;Ng LF;Chee RE;Stanton LW;Long PM;Liu ET
通讯作者：
Liu ET