Motilin in human milk: identification and stability during digestion.
Motilin in human milk: identification and stability during digestion.
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人乳中的胃动素：消化过程中的鉴定和稳定性。
DOI：
10.1016/s0024-3205(98)00477-9
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发表时间：
1998
期刊：
影响因子：
6.1
通讯作者：
T. Peeters
中科院分区：
文献类型：
--
作者：
P. De Clercq;S. Springer;I. Depoortere;T. Peeters
研究了人乳中胃动素的存在以及人乳对胃和十二指肠液降解[125 I][Nle 13]猪胃动素的影响。收集14名母亲的乳汁和血浆样本，采用放射免疫法测定胃动素。血浆水平为416 ± 37 pg/mL。8份脱脂样品胃动素水平为105 ± 14 pg/mL，6份高于1000 pg/mL，但稀释曲线呈非线性。固相萃取后，13份样品的乳汁水平为108 ± 21 pg/mL，1份样品的稀释曲线仍为非线性。通过将[125 I][Nle 13]猪胃动素与从新生儿获得的胃液和肠液（10倍稀释）孵育，在体外研究了胃动素摄入后的稳定性。在pH 1.8、3.2和5.8（胃液）和pH 7.4（十二指肠液）条件下，在37 ℃下进行孵育。在不同的时间间隔（5、10、20和30分钟）后，用三氯乙酸沉淀完整的胃动素，并测定上清液的放射性。胃动素被胃液迅速降解。在pH 3.2时分解最大（30分钟后为74%），在pH 5.8时分解最低（29%），即新生儿母乳喂养后的pH值。在pH 7.4的肠液中降解也非常迅速（30分钟后77%）。在pH 3.2时，人乳和BSA部分抑制胃消化（分别为17%和29%）。肠液消化不受母乳和BSA的影响。这些结果表明，胃胃动素的消化可能会充分延迟母乳在新生儿发挥生物学作用。
The presence of motilin in human milk and the influence of human milk on the degradation of [125I][Nle13] porcine motilin by gastric and duodenal fluids were investigated. Milk and plasma samples were collected from 14 mothers, and motilin was measured by radioimmunoassay. Plasma levels were 416 ± 37 pg/mL. In 8 defatted samples the motilin level was 105 ± 14 pg/mL, in the six others levels were above 1000 pg/mL but dilution curves were non-linear. After solid-phase extraction milk levels were 108 ± 21 pg/mL in 13 samples, in 1 sample the dilution curve was still non-linear. The stability of motilin after ingestion was studied in vitro by incubating [125I][Nle13] porcine motilin with gastric and intestinal juices obtained from newborns (10 times diluted). Incubations were performed at 37 °C at pH 1.8, 3.2 and 5.8 for the gastric fluid and at pH 7.4 for the duodenal fluid. After different times of intervais (5, 10, 20 and 30 minutes) intact motilin was precipitated with trichloroacetic acid and the radioactivity of the supernatant was determined. Motilin was rapidly degraded by gastric juice. The breakdown was greatest at pH 3.2 (74 % after 30 minutes) and lowest at pH 5.8 (29 %), the pH after milk feeding in neonates. Degradation by intestinal juice at pH 7.4 was also very rapid (77 % after 30 minutes). Human milk and BSA inhibited partially the gastric digestion at pH 3.2 (17 and 29 %, respectively). Digestion by intestinal juice was not affected by human milk and BSA. These results suggest that digestion of motilin in the stomach may be sufficiently retarded by human milk in the newborn to exert a biological role.
影响因子：
7.1
作者：
NEVILLE, MC;KELLER, R;ARCHER, P
通讯作者：
ARCHER, P
管腔蛋白质消化的发展：对生物活性膳食多肽的影响。
DOI：
10.1097/00005176-198908000-00004
发表时间：
1989
影响因子：
2.9
作者：
Britton,JR;Koldovsky,O
通讯作者：
Koldovsky,O
早产儿胃液对表皮生长因子的最小水解。
影响因子：
24.5
作者：
Britton,JR;George-Nascimento,C;Udall,JN;Koldovský,O
通讯作者：
Koldovský,O