Sampling strategies for wastewater surveillance: Evaluating the variability of SARS-COV-2 RNA concentration in composite and grab samples.
Sampling strategies for wastewater surveillance: Evaluating the variability of SARS-COV-2 RNA concentration in composite and grab samples.
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DOI：
10.1016/j.jece.2022.107478
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发表时间：
2022-06
影响因子：
7.7
通讯作者：
de Freitas Bueno R
中科院分区：
文献类型：
--
作者：
Augusto MR;Claro ICM;Siqueira AK;Sousa GS;Caldereiro CR;Duran AFA;de Miranda TB;Bomediano Camillo LM;Cabral AD;de Freitas Bueno R
受感染的人，甚至是无症状和少症状的人，会产生SARS-CoV-2RNA滴度，这使得可以使用废水监测来跟踪新冠肺炎在社区中的传播。这种方法很有趣，尤其是对于临床测试能力有限的新兴国家。然而，仍有一些重要的方法学方面需要验证，以使废水监测数据变得更具代表性和对公众健康更有用。这项研究评估了来自三个不同采样点的24小时合成和抓取样本中SARS-CoV-2RNA浓度的日间和日内变异性，其中包括两个污水处理厂(WTTP)和一个下水道沙井。在日间评估(17周监测)中，观察到每种采样方法的SARS-CoV-2RNA浓度之间有很好的一致性。考虑到N1和N2基因检测，Grab和复合样本的平均浓度之间没有显著差异(p值>；0.05)。相关系数证明了复合样品和抓取样品之间存在很强的相关性：皮尔逊相关系数为0.83，斯皮尔曼相关系数为0.78(p-Value<；0.05)。在日内评估中，分析了24小时周期，并观察到三个采样点每小时病毒浓度的低变异性。变异系数(CV)在3.0%~11.5%之间。总体而言，24小时图谱显示，病毒RNA浓度的变异性较小，与上午8点之间的平均值更接近。上午10点，建议抓取样本的时间。因此，本研究为新冠肺炎监测提供了废水采样技术方面的重要信息。只有在我们通过最佳做法确保数据质量的情况下，废水监测信息才会对公众健康和决策者有用。
The shedding of SARS-CoV-2 RNA titers by infected individuals, even asymptomatic and oligosymptomatic ones, allows the use of wastewater monitoring to track the COVID-19 spread in a community. This approach is interesting especially for emerging countries with limited clinical testing capabilities. However, there are still important methodological aspects that need validation so that wastewater monitoring data become more representative and useful for public health. This study evaluated the between-day and within-day variability of SARS-CoV-2 RNA concentrations in 24-hour composite and grab samples from three different sampling points, including two wastewater treatment plants (WTTP) and a sewer manhole. In the between-day evaluation (17 weeks of monitoring), a good agreement between the SARS-CoV-2 RNA concentration of each sampling method was observed. There were no significant differences between the mean concentrations of the grab and composite samples (p-value > 0.05), considering N1 and N2 gene assays. The strong relationship between composite and grab samples was proven by correlation coefficients: Pearson's r of 0.83 and Spearman's rho of 0.78 (p-value < 0.05). In within-day evaluation, 24-hour cycles were analyzed and low variability in hourly viral concentrations was observed for three sampling points. The coefficient of variation (CV) values ranged from 3.0% to 11.5%. Overall, 24-hour profiles showed that viral RNA concentrations had less variability and greater agreement with the mean values between 8 a.m. and 10 a.m, the recommended time for grab sampling. Therefore, this study provides important information on wastewater sampling techniques for COVID-19 surveillance. Wastewater monitoring information will only be useful to public health and decision-makers if we ensure data quality through best practices.
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