Proteomic analysis of Trichinella spiralis muscle larval excretory-secretory proteins recognized by early infection sera.
Proteomic analysis of Trichinella spiralis muscle larval excretory-secretory proteins recognized by early infection sera.
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早期感染血清识别的旋毛虫肌肉幼虫排泄蛋白的蛋白质组学分析
DOI：
10.1155/2013/139745
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发表时间：
2013
影响因子：
--
通讯作者：
Cui J
中科院分区：
文献类型：
--
作者：
Wang L;Wang ZQ;Hu DD;Cui J
虽然旋毛虫肌幼虫的排泄分泌（ES）蛋白是旋毛虫病最常用的诊断抗原，但其主要缺点是在感染早期的假阴性结果以及其主要组分（43、45、49和53 kDa）与其他蠕虫病患者血清的交叉反应。本研究的目的是寻找T. spiralis ES蛋白分子量为30-40 kDa。通过双向电泳（2-DE）对ES蛋白进行分析，共检测到约150个蛋白质点，等电点（pI）为4 ~ 7，分子量为14 ~ 66 kDa。当用感染后18天的感染小鼠的血清探测时，通过MALDI-TOF/TOF-MS识别并鉴定了10个分子量为30-40 kDa的蛋白质点。所有10个蛋白质点都被成功鉴定并表征为与5种不同的蛋白质相关，包括两种潜在的丝氨酸蛋白酶，一种保护性抗体靶向的抗原，一种脱氧核糖核酸酶（DNase）II，和一种保守的假设蛋白质这些蛋白可能是旋毛虫病的早期特异性诊断抗原。
Although the excretory-secretory (ES) proteins of Trichinella spiralis muscle larvae are the most commonly used diagnostic antigens for trichinellosis, their main disadvantage is the false negative results during the early stage of infection and cross-reaction of their main components (43, 45, 49, and 53 kDa) with sera of patients with other helminthiasis. The aim of this study was to identify early specific diagnostic antigens in T. spiralis ES proteins with 30–40 kDa. The ES proteins were analyzed by two-dimensional electrophoresis (2-DE), and a total of approximately 150 proteins spots were detected with isoelectric point (pI) varying from 4 to 7 and molecular weight from 14 to 66 kDa. When probed with sera from infected mice at 18 days postinfection, ten protein spots with molecular weight of 30–40 kDa were recognized and identified by MALDI-TOF/TOF-MS. All of ten spots were successfully identified and characterized to correlate with five different proteins, including two potential serine proteases, one antigen targeted by protective antibodies, one deoxyribonuclease (DNase) II, and one conserved hypothetical protein. These proteins might be the early specific diagnostic antigens for trichinellosis.
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影响因子：
1.5
作者：
Hewitson, James P.;Grainger, John R.;Maizels, Rick M.
通讯作者：
Maizels, Rick M.
影响因子：
2
作者：
Bien J;Näreaho A;Varmanen P;Gozdzik K;Moskwa B;Cabaj W;Nyman TA;Savijoki K
通讯作者：
Savijoki K
影响因子：
3.9
作者：
BARRY, MA;EASTMAN, A
通讯作者：
EASTMAN, A
影响因子：
2.6
作者：
Dea-Ayuela, MA;Bolás-Fernández, F
通讯作者：
Bolás-Fernández, F
影响因子：
2.4
作者：
Bolas-Fernandez, F.;del Corral Bezara, L.
通讯作者：
del Corral Bezara, L.