Creation, validation, and quantitative analysis of protein expression in vascular tissue microarrays.
Creation, validation, and quantitative analysis of protein expression in vascular tissue microarrays.
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DOI：
10.1016/j.carpath.2008.12.007
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发表时间：
2010-05
影响因子：
3.7
通讯作者：
Selvin, Elizabeth
中科院分区：
文献类型：
--
作者：
Halushka, Marc K.;Cornish, Toby C.;Lu, Jie;Selvin, Steve;Selvin, Elizabeth
生成了 17 个包含 1,200 多个血管组织的组织微阵列 (TMA)，以对人体血管系统进行全球调查。我们通过将这些代表性切片中的疾病特征与较大血管进行比较并通过证明重复切片中 3 种独立的免疫组织化学抗体染色的相关性来验证这些 TMA。组织微阵列 (TMA) 是平行放置在单个受体块中的多个组织核心的集合，传统上用于研究肿瘤组织中的蛋白质表达。我们验证了使用 TMA 来研究血管段中的蛋白质表达。从 100 名接受尸检的成年受试者身上采集血管组织。收获了一组不同的容器并排列在 17 个 TMA 上。使用组织化学和免疫组织化学 (IHC) 二氨基联苯胺 (DAB) 方法分析了 1,377 个独特的组织，每个组织的特征尺寸为 1.5 mm。血管疾病的组织形态计量学分析表明，TMA 特征捕获了原始血管切片中看到的大部分血管改变（内膜增生和动脉粥样硬化）。基于颜色解卷积的 IHC 染色强度测量用于量化指定感兴趣区域 (ROI) 中的抗原丰度。使用结缔组织生长因子 (CTGF)、晚期糖基化终末产物受体 (AGER/RAGE) 和基质金属蛋白酶 3 (MMP-3) 的抗体进行验证。 IHC 染色在这三种蛋白质的同一血管部位的重复特征之间高度相关。本研究验证了 TMA 技术利用染色强度数据研究血管壁的用途。
Seventeen tissue microarrays (TMAs) were generated containing over 1,200 vascular tissues to create a global survey of human vasculature. We validate these TMAs by comparing the disease characteristics in these representative sections to the larger vessels and through demonstrating correlations in staining for 3 separate immunohistochemical antibodies in duplicate sections. Tissue microarrays (TMAs) are collections of multiple tissue cores placed in parallel in a single acceptor block and traditionally used to investigate protein expression in neoplastic tissues. We validated the use of TMAs to investigate protein expression in vascular segments. Vascular tissues were collected from 100 adult subjects undergoing autopsy. A diverse set of vessels were harvested and arrayed over 17 TMAs. 1,377 unique tissues, each with a 1.5 mm feature size were analyzed using histochemical and immunohistochemical (IHC) diaminobenzidine (DAB) methods. Histomorphometric analysis of vascular disease demonstrated the TMA features captured the majority of the vascular alterations (intimal hyperplasia and atherosclerosis) seen in the original blood vessel section. Measurements of IHC staining intensity based on color deconvolution were used to quantify antigen abundance in defined regions of interest (ROI). Validation was performed using antibodies to connective tissue growth factor (CTGF), receptor for advanced glycation end products (AGER/RAGE) and matrix metalloproteinase 3 (MMP-3). IHC staining was highly correlated between duplicate features from the same vascular site over these three proteins. This study validates the use of TMA technology to investigate the vascular wall utilizing staining intensity data.
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影响因子：
--
作者：
Sun, Hua;Zheng, Jing-min;Li, Lei-shi
通讯作者：
Li, Lei-shi
影响因子：
82.9
作者：
Kononen, J;Bubendorf, L;Kallioniemi, OP
通讯作者：
Kallioniemi, OP
影响因子：
37.8
作者：
Fleiner, M;Kummer, M;Biedermann, BC
通讯作者：
Biedermann, BC
影响因子：
3.3
作者：
PELSTRING, RJ;ALLRED, DC;BANKS, PM
通讯作者：
BANKS, PM
影响因子：
2.8
作者：
Faith, DA;Isaacs, WB;De Marzo, AM
通讯作者：
De Marzo, AM