A general lack of compensation for gene dosage in yeast.
A general lack of compensation for gene dosage in yeast.
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DOI：
10.1038/msb.2010.19
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发表时间：
2010-05-11
影响因子：
9.9
通讯作者：
中科院分区：
文献类型：
--
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在人类、相关物种之间和不同的癌症组织中已经发现了基因拷贝数的变异，但目前还不清楚这种基因组水平的变异有多少会导致蛋白质丰度水平的变化。为了解决这个问题，我们消除了酿酒酵母中730个不同基因的两个基因组拷贝之一，并询问基因剂量减少50%导致蛋白质水平减少50%的频率。对于至少80%的测试基因，在几种环境条件下，它确实：杂合菌株中的蛋白质水平接近野生型的50%。对于<5%的测试基因，杂合子中的蛋白质水平维持在接近野生型水平。这些实验表明，蛋白质水平一般不直接监测和调节至所需水平。结合适应性数据，这意味着蛋白质的表达水平高于生存所需的水平。
Gene copy number variation has been discovered in humans, between related species, and in different cancer tissues, but it is unclear how much of this genomic-level variation leads to changes in the level of protein abundance. To address this, we eliminated one of the two genomic copies of 730 different genes in Saccharomyces cerevisiae and asked how often a 50% reduction in gene dosage leads to a 50% reduction in protein level. For at least 80% of genes tested, and under several environmental conditions, it does: protein levels in the heterozygous strain are close to 50% of wild type. For <5% of the genes tested, the protein levels in the heterozygote are maintained at nearly wild-type levels. These experiments show that protein levels are not, in general, directly monitored and adjusted to a desired level. Combined with fitness data, this implies that proteins are expressed at levels higher than necessary for survival.
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影响因子：
56.9
作者：
Hillenmeyer, Maureen E.;Fung, Eula;Giaever, Guri
通讯作者：
Giaever, Guri
影响因子：
4.5
作者：
Preker, PJ;Kim, KS;Guthrie, C
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Guthrie, C
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64.8
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Dekel, E;Alon, U
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Alon, U
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64.5
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Mnaimneh, S;Davierwala, AP;Hughes, TR
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Hughes, TR
影响因子：
64.5
作者：
PEARSON, NJ;FRIED, HM;WARNER, JR
通讯作者：
WARNER, JR