The Evaluation of a Low-Cost Colorimeter for Glucose Detection in Salivary Samples.
The Evaluation of a Low-Cost Colorimeter for Glucose Detection in Salivary Samples.
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DOI：
10.3390/s17112495
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发表时间：
2017-11-01
期刊：
影响因子：
--
通讯作者：
Márquez-Lucero A
中科院分区：
文献类型：
--
作者：
Dominguez RB;Orozco MA;Chávez G;Márquez-Lucero A
鉴于获取医疗资源受限，低收入环境需要开发平价技术。在此，我们介绍一种低成本比色计的设计与评估，该比色计通过检测唾液中的葡萄糖，应用于糖尿病的无创监测。样本通过葡萄糖氧化酶 - 过氧化物酶酶系统处理，并使用研发的设备进行分析。采用532.5nm的发光二极管作为激发源，RGB模块作为接收器。通过将记录的RGB成分与葡萄糖浓度相关联，绘制出定量唾液葡萄糖浓度（0至18mg/dL）的校准曲线，检测限达到0.17mg/dL，变异系数为5%（n = 3）。使用该设备处理糖尿病患者和健康志愿者的唾液样本，结果显示前者平均浓度为1.5519 ± 0.4511mg/dL，后者为4.0479 ± 1.6103mg/dL，从而能够区分两组。结果通过紫外 - 可见 - 近红外分光光度计进行验证，两种仪器之间的R²对应值为0.98194。结果表明，所研发的设备有可能应用于以低成本、用户友好、低功耗且便携的仪器灵敏检测相关分析物。
Given the limited access to healthcare resources, low-income settings require the development of affordable technology. Here we present the design and evaluation of a low-cost colorimeter applied to the non-invasive monitoring of Diabetes Mellitus through the detection of glucose in salival fluid. Samples were processed by the glucose oxidase-peroxidase enzymatic system and analyzed with the development equipment. A light emission diode of 532.5 nm was used as an excitation source and a RGB module was used as a receptor. A calibration curve to quantify the concentration of salivary glucose (0 to 18 mg/dL) was carried out by relating the RGB components registered with glucose concentrations, achieving a limit of detection of 0.17 mg/dL with a CV of 5% (n = 3). Salivary samples of diabetic and healthy volunteers were processed with the equipment showing an average concentration of 1.5519 ± 0.4511 mg/dL for the first and 4.0479 ± 1.6103 mg/dL for the last, allowing a discrimination between both groups. Results were validated against a UV-Vis-NIR spectrophotometer with a correspondence of R2 of 0.98194 between both instruments. Results suggest the potential application of the developed device to the sensitive detection of relevant analytes with a low-cost, user-friendly, low-power and portable instrumentation.
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影响因子：
7.4
作者：
Nery, Emilia Witkowska;Kundys, Magdalena;Jonsson-Niedziolka, Martin
通讯作者：
Jonsson-Niedziolka, Martin
影响因子：
3.7
作者：
Mascarenhas P;Fatela B;Barahona I
通讯作者：
Barahona I
影响因子：
8.4
作者：
Bueno Hernandez, Diana;Mishra, Rupesh K.;Marty, Jean Louis
通讯作者：
Marty, Jean Louis
影响因子：
3.3
作者：
Green, Michael D.;Hostetler, Dana M.;Newton, Paul N.
通讯作者：
Newton, Paul N.
影响因子：
--
作者：
Balan P;Babu SG;Sucheta KN;Shetty SR;Rangare AL;Castelino RL;Fazil AK
通讯作者：
Fazil AK