The protective effect of selenium on the lipopolysaccharide-induced oxidative stress and depressed gene expression related to milk protein synthesis in bovine mammary epithelial cells
The protective effect of selenium on the lipopolysaccharide-induced oxidative stress and depressed gene expression related to milk protein synthesis in bovine mammary epithelial cells
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硒对脂多糖诱导的牛乳腺上皮细胞氧化应激和乳蛋白合成相关基因表达抑制的保护作用
DOI：
10.1007/s12011-019-01961-7
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发表时间：
2019-11
影响因子：
3.9
通讯作者：
Shi Binlin
中科院分区：
文献类型：
--
作者：
Zhang Boqi;Guo Yongmei;Yan Sumei;Guo Xiaoyu;Zhao Yanli;Shi Binlin
本研究旨在探讨硒对牛乳腺上皮细胞（BMEC）抗氧化功能和乳蛋白合成的影响。两个实验采用单因素完全随机设计研究。在第一部分中，BMEC被随机分为7组：对照组（不含硒）和6个硒处理组（10，20，50，100，150和200 nmol/L）。第二部分在第一部分研究的基础上，以脂多糖（LPS）为诱导应激源，分析硒对LPS诱导的BMEC氧化损伤的保护作用及对BMEC乳蛋白合成的影响。将BMEC随机分为8组：对照组（不加Se和LPS）、LPS处理组（仅加LPS）和6个Se加LPS处理组（LS 10 ~ LS 200）。用硒处理BMEC被发现显着提高细胞增殖和抗氧化功能。LPS可诱导BMEC氧化损伤，显著抑制细胞增殖和抗氧化功能。硒对LPS诱导的BMEC氧化损伤具有保护作用。此外，我们的研究结果表明，LPS损伤下调乳蛋白合成的基因表达。硒能有效缓解LPS诱导的氧化损伤对乳蛋白合成的抑制作用，且硒浓度在50 ~ 200 nmol/L时效果最好。总之，硒在50至100 nmol/L的浓度是更好的抗氧化功能，但对牛奶蛋白质合成的健康BMEC没有影响。硒通过提高BMEC中抗氧化标记物的水平、上调乳蛋白合成和乳蛋白相关基因的表达来改善LPS诱导的BMEC损伤。
The objective of this study was to determine the effects of selenium (Se) on antioxidative function and the synthesis of milk protein in bovine mammary epithelial cells (BMECs). Two experiments were conducted using a single-factor completely randomized design study. In part I, BMECs were randomly divided into seven groups: control (without Se) and six Se treatments (10, 20, 50, 100, 150, and 200 nmol/L). In part II, based on the results of part I, we used lipopolysaccharide (LPS) as the induced stress source to analyze the protective effect of Se on LPS-induced oxidative damage and the influence on milk protein synthesis of BMECs. BMECs were randomly divided into eight groups: control (without Se and LPS), LPS treatment (only LPS), and six Se treatments with LPS (LS10 to LS200). Treatment of BMECs with Se was found to significantly improve cell proliferation and antioxidant function. LPS could induce oxidative damage which significantly inhibited cell proliferation and antioxidant function in BMECs. Se had a protective effect on the oxidative damage of BMECs induced by LPS. Additionally, our results indicated that LPS damage downregulated the gene expression of milk protein synthesis. Se effectively relieved the inhibition due to LPS-induced oxidative damage on the synthesis of milk protein, and Se concentrations of 50 to 200 nmol/L showed the best effect. In conclusion, Se at concentrations of 50 to 100 nmol/L is better for antioxidant function but had no effect on milk protein synthesis in healthy BMECs. Se ameliorated the damage caused by LPS-induced by improving levels of antioxidant markers and upregulating milk protein synthesis and the expression of genes associated with milk protein in BMECs.
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影响因子：
3.5
作者：
Ametaj, B. N.;Emmanuel, D. G. V.;Dunn, S. M.
通讯作者：
Dunn, S. M.
影响因子：
3.9
作者：
Gao, Xuejiao;Zhang, Zecai;Zhang, Naisheng
通讯作者：
Zhang, Naisheng
影响因子：
5.1
作者：
Zhang, Wen;Zhang, Runxiang;Zhang, Naisheng
通讯作者：
Zhang, Naisheng
影响因子：
5.1
作者：
Zhengkai Wei;Minjun Yao;Yimeng Li;Xuexiu He;Zhengtao Yang
通讯作者：
Zhengkai Wei;Minjun Yao;Yimeng Li;Xuexiu He;Zhengtao Yang
影响因子：
5.1
作者：
Zhengkai Wei;Minjun Yao;Yimeng Li;Zhengtao Yang;Xiaosheng Feng
通讯作者：
Zhengkai Wei;Minjun Yao;Yimeng Li;Zhengtao Yang;Xiaosheng Feng